The Nonstructural Protein 1 Papain-Like Cysteine Protease Was Necessary for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 to Inhibit Interferon-beta Induction
文献类型： 外文期刊
作者： Shi, Xibao 1 ; Zhang, Gaiping 1 ; Wang, Li 1 ; Li, Xuewu 1 ; Zhi, Yubao 1 ; Wang, Fangyu 1 ; Fan, Jianming 1 ; Deng, Ruig 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Zhengzhou Univ, Coll Publ Hlth, Toxicol Lab, Zhengzhou, Peoples R China
关键词： nonstructural protein-1;papain-like cysteine protease: 37353-41-6;interferon-beta: IFN-beta;74899-71-1;production;induction;nonstructural protein-1-alpha;nonstructural protein-1-beta;enzyme activity
期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）
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收录情况： SCI
摘要： Porcine reproductive and respiratory syndrome virus nonstructural protein 1 (nsp1) could be auto-cleaved into nsp1 alpha and nsp1 beta, both of which had the papain-like cysteine protease activities. Previous studies have shown that porcine reproductive and respiratory syndrome virus nsp1 was an interferon (IFN) antagonist. However, the mechanism by which nsp1 inhibited IFN-beta production was unclear. Here, we used site-directed mutagenesis that inactivated the papain-like cysteine protease activities of nsp1 to explore whether the papain-like cysteine protease activities were required for nsp1 to disrupt IFN-beta production. The results showed that mutations that inactivated papain-like cysteine protease activity of nsp1 alpha made nsp1 lose its IFN antagonism activity, whereas mutations that inactivated papain-like cysteine protease activity of nsp1 beta did not influence the IFN antagonism activity of nsp1. In conclusion, our present work indicated that the papain-like cysteine protease activity of nsp1 alpha was necessary for nsp1 to inhibit IFN-beta induction.
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