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Contribution of different class 2 integron elements to fitness costs in multi-drug resistant Escherichia coli and evaluation of their adaptability in ?farm-to-table? environments

文献类型： 外文期刊

作者： Jiang, Han 1 ; Jiao, Xue 1 ; Yu, Ting 1 ; Wang, Wen 2 ; Cheng, Hui 4 ; Huang, Guangrong 1 ; Fang, Jiehong 1 ;

作者机构： 1.China Jiliang Univ, Coll Life Sci, Key Lab Specialty Agriprod Qual & Hazard Controlli, Hangzhou 310018, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou 310021, Zhejiang, Peoples R China

4.Hangzhou Wahaha Grp Co Ltd, Res & Develop Dept, Hangzhou 310018, Zhejiang, Peoples R China

关键词： Typical class 2 integron; Fitness cost; Adaptability; E; coli; Farm-to-table

期刊名称：FOOD MICROBIOLOGY （ 影响因子：5.3； 五年影响因子：5.5 ）

ISSN： 0740-0020

年卷期： 2023 年 113 卷

页码：

收录情况： SCI

摘要： Integrons play a pivotal role in the dissemination of antimicrobial resistance, because they can capture and express exogenous antimicrobial resistance genes. This study aimed to elucidate the structure and contribution of different elements of class 2 integrons to fitness costs in their host bacteria and evaluate their adaptability to the "farm-to-table" process. We mapped 27 typical class 2 integrons of Escherichia coli isolated from aquatic foods and pork products, each harboring an inactive truncated class 2 integrase gene and the gene cassette (GC) array dfrA1-sat2-aadA1 with strong Pc2A/Pc2B promoters. Notably, the fitness costs associated with class 2 integrons depended on the Pc promoter strength and quantity and content of GCs in the array. Additionally, the costs of integrases were activity-dependent, and a balance was identified between GC capture ability and integron sta-bility, which could explain the inactive truncated integrase identified. Although typical class 2 integrons exhibited low-cost structures in E. coli, the bacteria incurred biological costs, including decreasing growth rates and biofilm formation, in farm-to-table environments, especially under low-nutrient conditions. Nevertheless, sub-inhibitory antibiotic concentrations led to the selection of class 2 integron-carrying bacteria. This study provides important insights into how integrons may travel from preharvest to consumer goods.

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