Inoculation of thermophilic bacteria from giant panda feces into cattle manure reduces gas emissions and decreases resistance gene prevalence in short-term composting
文献类型： 外文期刊
作者： Fu, Jingxia 1 ; Chen, Shujuan 3 ; Tan, Yulan 2 ; Zou, Keyi 2 ; Yu, Xiumei 2 ; Ji, Lin 2 ; Zhang, Jianmin 5 ; Xiao, Xingning 6 ; Wang, Wen 6 ; Zhao, Ke 2 ; Zou, Likou 2 ;
作者机构： 1.Sichuan Agr Univ, Coll Environm Sci, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Coll Resources, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Coll Food Sci, Chengdu 611130, Sichuan, Peoples R China
4.Guizhou Normal Univ, Sch Life Sci, Guiyang 550025, Guizhou, Peoples R China
5.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
6.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, MOA Lab Qual & Safety Risk Assessment Agroprod Han, Hangzhou 310021, Peoples R China
关键词： Aerobic composting; Thermophilic bacteria; Greenhouse gas; Resistance genes
期刊名称：JOURNAL OF ENVIRONMENTAL MANAGEMENT （ 影响因子：8.4； 五年影响因子：8.6 ）
ISSN： 0301-4797
年卷期： 2025 年 373 卷
页码：
收录情况： SCI
摘要： Here, thermophilic bacteria (TB) with cellulose degradation functions were screened from composting panda feces and applied to cattle manure composting. TB (Aeribacillus pallidus G5 and Parageobacillus toebii G12) inoculation led to remarkable improvement of the compost temperature, prolonging of the thermophilic stage and shortening of the composting process, resulting in increased manure harmlessness (GI >= 70%), compost humification, and greenhouse gas emission reduction (14.19%-22.57%), compared with the control compost, within 15 days of composting. In particular, G5 inoculation reduced NH3 emissions by 41.97% relative to control composts over 15 days. G5 was capable of rapidly colonizing in the composts, and its inoculation immediately enriched the genera of Firmicutes, and simultaneously decreased the genera of Proteobacteria, contributing to the elimination of harmful microorganisms. Notably, this strain lacked antibiotic resistance genes, and the absolute abundances of resistance genes and mobile genetic genes (MGEs) decreased the most (by 80.84%). Metagenomic analysis revealed that enzymes capable of producing CO2, N2O, and NH3 were generally inhibited, while CO2 fixation and N2O and NH3 reduction enzymes were enriched in the G5 compost, since metagenomeassembled genomes of Proteobacteria harbored more key genes and enzymes in complete pathways for producing N2O, NH3, and CO2. Moreover, Proteobacteria, such as Pseudomonas and Halopseudomonas, were the main host of resistance genes and MGEs. Overall, the gas emission could be reduced, and more efficient control of resistance genes could be achieved by inhibited the abundance of Proteobacteria during composting. This study provides a safe and effective microbial agent (A. pallidus) for manure treatment.
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