您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Exploration and molecular mechanism of novel ACE inhibitory peptides from goat milk protein: A combined in silico and in vitro study

文献类型： 外文期刊

作者： Zhu, Ruikai 1 ; Zhang, Hong 2 ; Wu, Yulong 1 ; Li, Huanhuan 2 ; Tang, Honggang 2 ; Xu, Xianrong 1 ; Zhao, Ke 2 ;

作者机构： 1.Hangzhou Normal Univ, Sch Publ Hlth, Hangzhou 311121, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Zhejiang Key Lab Intelligent Food Logist & Proc, Hangzhou 310021, Peoples R China

3.Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Xian 710062, Peoples R China

关键词： Goat milk protein; ACE inhibitory peptide; Enzymatic hydrolysis; Virtual screening; Molecular docking

期刊名称：INTERNATIONAL DAIRY JOURNAL （ 影响因子：3.4； 五年影响因子：3.6 ）

ISSN： 0958-6946

年卷期： 2025 年 167 卷

页码：

收录情况： SCI

摘要： Hypertension poses a significant threat to human health, and the efficient exploration of food-derived angiotensin I-converting enzyme inhibitory (ACEI) peptides as milder and safer alternatives to ACEI drugs has garnered increasing attention. Therefore, a combined in silico and in vitro study was performed to investigate novel ACEI peptides from goat milk proteins. First, the papain plus proteinase K was identified as the best enzymatic combination for generating ACEI peptides using BIOPEP. Then the enzymatic conditions of the papain/proteinase, the input order of the enzymes, and the molecular weight (MW) of the hydrolysate were further optimized to obtain the hydrolysate with the highest ACEI activity. Under the optimized condition described, 698 peptides (<25 A A) were successfully identified from the milk hydrolysate of goat (MW < 3 KDa). Among them, six potential novel ACEI peptides were efficiently screened virtually. Verification studies using synthesized peptides partially confirmed the in silico results, with four out of the six selected peptides (FKF, FRY, RWL, and WKP) exhibiting ACEI activity. Additionally, the blood pressure-lowering effects of the top two ACEI peptides (FRY and FKF) were further validated in spontaneously hypertensive rats (SHRs), and FRY treatment significantly lowered the blood pressure of SHRs. To summarize, this study established a rapid enzymatic hydrolysis system for screening novel ACEI peptides and identified a novel antihypertensive peptide, FRY, which provides a new method for research on bioactive peptides research and high-value utilization of goat milk.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台