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Molecular characteristics and expression profiles of glycerol-3-phosphate dehydrogenase 1 (GPD1) gene in pig

文献类型： 外文期刊

作者： Gao, Y. Z. 1 ; Jiang, Y. 1 ; Wu, X. 2 ; Bai, C. Y. 1 ; Pan, Y. C. 1 ; Sun, Y. Z. 3 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Anim Sci, Shanghai 200240, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai 201106, Peoples R China

3.Guizhou Univ, Coll Agr, Guiyang 550025, Guizhou, Peoples R China

关键词： Glycerol-3-phosphate dehydrogenase 1;Clone;Mapping;Real-Time PCR;Pig

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： The cytosolic activity of glycerol-3-phosphate dehydrogenase 1 (GPD1, EC 1.1.1.8) plays an important role in the synthesis of triacylglycerol and in the transport of reducing equivalents from the cytosol to mitochondria. Here we report the full-length genomic sequence of porcine GPD1 gene including promoter region. Porcine GPD1 gene contains eight exons and seven introns. Using the ImpRH, the GPD1 gene was mapped on chromosome 5. Sub-cellular localization of the pig GPD1 was localized in cytoplasm by GFP reporter gene. The full-length CDS of porcine GPD1 gene comprises 1050 nucleotides and it encodes 349 amino acids. Using the CDS sequences of 17 species, we built the phylogeny tree of GPD1 gene. We investigated the expression level of the gene in 13 different tissues and time course from birth to postnatal day28 in longissinus doris muscle (LD) and in cerebrum. The result shows that porcine GPD1 gene is expressed in almost all tissues we tested but its levels of expression varies widely over 2 orders of magnitude. LD and the cerebrum have similar expression pattern that is at a low level at birth and increasing with aging to the highest level at postnatal day8 in LD and postnatal day14 in cerebrum. But weaning decreased the expression level of the GPD1 gene. This may partially explains the effects of weaning on energy metabolism.

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