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Isolating, Mapping and Spatio-Temporal Distribution Analysis of Five Ubiquitin-Specific Protease Genes in Porcine

文献类型： 外文期刊

作者： Wu, X. 1 ; Zhang, X. B. 3 ; Zhu, L. L. 1 ; Tan, Y. S. 1 ; Tang, X. M. 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

2.Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

3.Huazhong Agr Univ, Sch Food Sci & Technol, Wuhan 430070, Peoples R China

关键词： Ubiquitin-specific protease;porcine;mapping;expreesion;big muscle tissue;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2011 年 10 卷 4 期

页码：

收录情况： SCI

摘要： The Ubiquitin-proteasome system is an essential mechanism for protein degradation in eukaryotes and ubiquitin-specific proteases are key effectors of the Ubiquitin-proteasome system. About 5 porcine USP genes (14, 16, 38, 44 and 54) were isolated and shared high sequence similarity with their human homologues genes. Then, the tissue expression pattern of 5 porcine USP genes were analysed by RT-PCR in 2 breeds (a domestic breed and a foreign breed) and the results showed that USP 14, 16, 38 and 54 gene expression were higher in muscle tissue. In addition, researchers mapped the 5 porcine genes and all chromosomes assignments were consistent with known chromosomal homologies between human and pig. All results provided a basis for further function study of these 5 USP genes.

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