文献类型： 外文期刊
作者： Li, P. 1 ; Yang, S. F. 1 ; Lv, B. B. 1 ; Zhao, K. 1 ; Lin, M. F. 1 ; Zhou, S. 3 ; Song, X. 3 ; Tang, X. M. 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotech Res Inst, Shanghai, Peoples R China
2.Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
3.Shanghai Acad Agr Sci, Inst ECO Environm & Plant Protect, Shanghai, Peoples R China
关键词： Extraction efficiency;Freshwater;Microbial diversity;Polymerase chain reaction;Total DNA
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 1 期
页码：
收录情况： SCI
摘要： The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water. DNA extraction was analyzed by agarose gel electrophoresis, ultraviolet-spectroscopy, restriction enzyme digestion, and polymerase chain reaction. The results showed that the modified filtration-membrane method was the most efficient for extracting microbial DNA from freshwater with high integrity and purity and is suitable for molecular applications.
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