A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers
文献类型： 外文期刊
作者： Shao, Qing-Song 1 ; Guo, Qiao-Sheng 1 ; Deng, Yan-Ming 3 ; Guo, Hai-Peng 2 ;
作者机构： 1.Nanjing Agr Univ, Inst Chinese Med Mat, Nanjing 210095, Peoples R China
2.Zhejiang A&F Univ, Hangzhou 311300, Zhejiang, Peoples R China
3.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）
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年卷期：
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收录情况： SCI
摘要： The diversity and genetic relationship among 29 populations of Chrysanthemum morifolium, one of Chrysanthemum indicum and one of Chrysanthemum nankingense from China were analyzed using morphological traits and molecular markers. Twenty morphological traits were scored as well as 182 ISSR marker-fragments, as amplified by 22 primers [the percentage of polymorphic bands (PPB): 81.87%], and 243 SRAP marker-fragments as generated by 26 primer pairs (PPB: 75.72%). Mantel's test indicated significant correlation (r = 0.624) of morphological trait and SRAP. By contrast, the morphological trait showed low correlation with ISSR (r = 0.246). Cluster analysis showed groupings of the accessions according to all four methods correlated well with their geographic region of origin, and most populations from the south of China were classified into one cluster and most populations from the north of China were classified into another cluster. Finally, an appropriate strategy for conserving the C. morifolium germplasm was proposed.
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