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Molecular detection of Pectobacterium species causing soft rot of Amorphophallus konjac

文献类型： 外文期刊

作者： Wu, Jinping 1 ; Diao, Ying 1 ; Gu, Yucheng 2 ; Hu, Zhongli 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, Minist Educ Plant Dev Biol, Key Lab, Wuhan 430072, Peoples R China

2.Hubei Acad Agr Sci, Ind Crops Inst, Wuhan 430064, Peoples R China

3.Chongqing Univ Sci & Arts, Coll Life Sci & Technol, Chongqing 402160, Peoples R China

关键词： Amorphophallus konjac;Real-time PCR;Soft rot disease;Pectobacterium species

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Soft rot disease of Amorphophallus konjac is caused by Pectobacterium species. Infected corms are considered a primary and important source of inocula. Based on the 16S rDNA sequences of the soft rot pathogens, one pair of specific primers was designed to identify the soft rot disease by real-time PCR and the other two were used to identify the pathogens of Pectobacterium carotovorum subsp. carotovorum. and P. chrysanthemi respectively. According to the results, a single cell of Pectobacterium could be detected by real-time PCR with the designed primer pair, while at least 100 bacteria were required for conventional PCR. Moreover, the two special primers can directly and accurately authenticate to Pectobacterium carotovorum subsp. carotovorum and P. chrysanthemi by the conventional PCR system without testing the pathogenicity, biochemical and phenotypic characterizations and so on. In conclusion, the PCR-based techniques showed several significant advantages in identifying the soft rot pathogens from konjac, such as higher sensitivity, rapidness and precision, and it could be widely used in seed quarantine.

  • 相关文献

[1]Infection pathways of soft rot pathogens on Amorphophallus konjac. Wu, Jinping,Diao, Ying,Hu, Zhongli,Wu, Jinping,Gu, Yucheng. 2010

[2]Authentication and characterization of a candidate antagonistic bacterium against soft rot of Amorphophallus konjac. Wu, Jinping,Diao, Ying,Hu, Zhongli,Wu, Jinping,Liu, Xiaoyan,Ding, Zili. 2012

[3]Analysis of bacterial communities in rhizosphere soil of continuously cropped healthy and diseased konjac. Wu, Jinping,Jiao, Zhenbiao,Zhou, Jie,Guo, Fengling,Ding, Zili,Qiu, Zhengming.

[4]TOLL样受体在疟疾感染小鼠模型中表达量变化规律的研究. 孙斐,许兵红,杨小林,张佑宏,张海珠. 2013

[5]转基因水稻多重PCR和Real-time PCR定性检测中的问题与策略. 仇娟. 2015

[6]一种分子检测魔芋中黄瓜花叶病毒的方法. 盛佳婧,钟琳,杨朝柱,张绍彬,谢吉容,吴金平,胡中立,刁英. 2015

[7]Imprinting analysis of porcine DIO3 gene in two fetal stages and association analysis with carcass and meat quality traits. Qiao, Mu,Guo, Ling,Zhang, Peng-Peng,Li, Feng-E,Zheng, Rong,Deng, Chang-Yan,Qiao, Mu,Guo, Ling,Zhang, Peng-Peng,Li, Feng-E,Zheng, Rong,Deng, Chang-Yan,Qiao, Mu,Wu, Hua-Yu,Mei, Shu-Qi,Wu, Hua-Yu,Mei, Shu-Qi.

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