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Characterization of a ranavirus isolated from cultured largemouth bass (Micropterus salmoides) in China

文献类型： 外文期刊

作者： Deng, Guocheng 1 ; Li, Shengjie 1 ; Xie, Jun 1 ; Bai, Junjie 1 ; Chen, Kunci 1 ; Ma, Dongmei 1 ; Jiang, Xiaoyan 2 ; Lao, 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fish Breeding & Cultivat, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fish Breeding & Cultivat,

关键词： Largemouth bass Micropterus salmoides;Pathogen;Ranavirus;Major capsid protein (MCP)

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

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收录情况： SCI

摘要： During June through October 2008, numerous cultured largemouth bass (Micropterus salmoides) died in the Foshan area of Guangdong Province, China. Affected fish had ulcerations on the skin and muscle. Epithelioma papillosum cyprini (EPC) cell cultures inoculated with filtrates of muscle homogenates from the diseased fish developed the cytopathic effect (CPE) 5days after inoculation at the primary passage. Transmission electron microscopy of the ulcerative muscle tissue and the infected EPC cells revealed a cytoplasmic, icosahedral virion that averaged 145nm in diameter. Intramuscular injection of the virus (a[control][yen107.82 TCID50 mla degree 1) resulted in clinical signs of the disease and caused 100% mortality of healthy largemouth bass. In addition, the mandarinfish (Siniperca chuatsi) was susceptible to the virus after intramuscular injection of the virus (0.2ml of 109.82 TCID50 mla degree 1), and the mortality was 20%. However, no death or clinical signs were found in grass carp (Ctenopharyngodon idella), bighead carp (Aristichthys nobilis), Nile tilapia (Oreochromis niloticus), crucian carp (Carassius carassius), common carp (Cyprinus carpio), and fancy carp (C. carpio) after experimental infection. DNA fragments of the viral major capsid protein (MCP) and DNA methyltransferase (MTase) were amplified and sequenced. Subsequent multiple alignment and phylogenetic analysis revealed that the virus is identical to doctor fish virus (DFV) and closely related to largemouth bass virus (LMBV).

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