A TMT-Based Proteomic Analysis of Osmoregulation in the Gills of Oreochromis mossambicus Exposed to Three Osmotic Stresses
文献类型： 外文期刊
作者： Su, Huanhuan 1 ; Ma, Dongmei 1 ; Fan, Jiajia 1 ; Zhong, Zaixuan 1 ; Tian, Yuanyuan 1 ; Zhu, Huaping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Trop & Subtrop Fishery Resource Applicat &, 1 Xingyu Rd, Guangzhou 510380, Peoples R China
2.Heyuan Branch, Guangdong Lab Lingnan Modern Agr, Heyuan 517000, Peoples R China
3.Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou 510380, Peoples R China
关键词：
TMT-based proteomics; gills; osmotic stresses;
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 6 期
页码：
收录情况： SCI
摘要： Salinity and alkalinity are critical environmental factors that affect fish physiology and ability to survive. Oreochromis mossambicus is a euryhaline species that can endure a wide range of salinities and has the potential to serve as a valuable model animal for environmental science. In order to detect the histomorphological changes, antioxidant enzymes, and proteomic responses of O. mossambicus to different osmotic stresses, O. mossambicus was subjected to salinity stress (25 g/L, S_S), alkalinity stress (4 g/L, A_S), saline-alkalinity stress (salinity: 25 g/L, alkalinity: 4 g/L, SA_S), and freshwater (the control group; C_S). The histomorphological and antioxidant enzyme results indicated that salinity, alkalinity, and saline-alkalinity stresses have different degrees of damage and effects on the gills and liver of O. mossambicus. Compared with the control, 83, 187, and 177 differentially expressed proteins (DEPs) were identified in the salinity, alkalinity, and saline-alkalinity stresses, respectively. The obtained DEPs can be summarized into four categories: ion transport channels or proteins, energy synthesis and metabolism, immunity, and apoptosis. The KEGG enrichment results indicated that DNA replication and repair were significantly enriched in the salinity stress group. Lysosomes and oxidative phosphorylation were considerably enriched in the alkalinity stress group. Comparatively, the three most important enriched pathways in the saline-alkalinity stress group were Parkinson's disease, Alzheimer's disease, and Huntington's disease. The findings of this investigation yield robust empirical evidence elucidating osmoregulatory mechanisms and adaptive biological responses in euryhaline teleost, thereby establishing a scientific foundation for the cultivation and genomic exploration of high-salinity-tolerant teleost species. This advancement facilitates the sustainable exploitation of saline-alkaline aquatic ecosystems while contributing to the optimization of piscicultural practices in hypersaline environments.
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