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Quantitative analysis of temperature, salinity and pH on WSSV proliferation in Chinese shrimp Fenneropenaeus chinensis by real-time PCR

文献类型： 外文期刊

作者： Gao, Huan 1 ; Kong, Jie 1 ; Li, Zhanjun 1 ; Xiao, Guangxia 1 ; Meng, Xianhong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China

关键词： Fenneropenaeus chinensis;Temperature;Salinity;pH;WSSV

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： White spot syndrome virus (WSSV) is the most serious disease threatening the shrimp farming. Previous studies have elucidated the relationships between WSSV outbreak and environmental factors such as temperature, salinity and pH. But, these studies didn't expatiate the relationships between the copy numbers of WSSV replication and the changes of environmental factors, even educed some contrary conclusions. Here, by the methods of Taguchi and real time PCR, the impact of three factors, temperature, salinity and pH on the proliferation of WSSV was studied together. The five levels selected for three factors were 15A degree C, 20A degree C, 25A degree C, 30A degree C and 35A degree C for temperature, 15a degree , 20a degree , 25a degree , 30a degree and 35a degree for salinity and 6.5, 7.5, 8.0, 8.5 and 9.0 for pH. ANOVA analysis showed that significant differences existed between 15A degree C and 25A degree C (P=0.044), between 20A degree C and 25A degree C (P=0.047), and between 35A degree C and 25A degree C (P=0.044) for temperature. No significant differences were found among the levels of salinity and pH. This discovered that, of the three factors, the temperature was a crucial factor in determining WSSV proliferation. Hyperthermia (35A degree C) and hypothermia (15A degree C) could significantly decrease the WSSV proliferation while 25A degree C was the optimum temperature of WSSV replication. Moreover, low temperature was a more efficient factor to control WSSV replication and mortality of individuals infected WSSV, because the copy numbers of WSSV were only 0.626 per ng muscle at 15A degree C, while there were 5.94 copies at 35A degree C. Our study showed that the optimal WSSV proliferation condition was 30A degree C for temperature, 35a degree for salinity, and 8.0 for pH, and reversely, the worst WSSV proliferation condition was 15A degree C for temperature, 35a degree for salinity, and 9.0 for pH.

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