Development of an indirect competitive enzyme-linked immunosorbent assay for propiconazole based on monoclonal antibody
文献类型： 外文期刊
作者： Li, Jiao 1 ; Ding, Yuan 1 ; Chen, He 1 ; Sun, Wanlin 1 ; Huang, Yue 1 ; Liu, Fengquan 1 ; Wang, Minghua 1 ; Hua, Xiude 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
2.State & Local Joint Engn Res Ctr Green Pesticide, Nanjing 210095, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
关键词： Propiconazole; Hapten; Monoclonal antibodies; Indirect competitive enzyme-linked immuno-; sorbent assay; Pesticide residues
期刊名称：FOOD CONTROL （ 影响因子：6.652； 五年影响因子：6.498 ）
ISSN： 0956-7135
年卷期： 2022 年 134 卷
页码：
收录情况： SCI
摘要： Propiconazole is a widely used fungicide in agricultural production, but it poses risks to human health. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) was established to detect propiconazole residues. Three haptens of propiconazole were designed and synthesized to prepare antigens. Ten mAbs were obtained using hapten B (5-(4-(2-((1H-1,2,4-triazol-1-yl)methyl)-4-propyl-1,3dioxolan-2-yl)-3-chlorophenyl) pentanoic acid). The ic-ELISA based on the combination of mAb 2G2E12 and hapten B-ovalbumin (coating antigen) showed the highest sensitivity. After optimization, the 50% inhibition concentration, limit of detection and linear range of the ic-ELISA were 2.33 mu g/L, 0.26 mu g/L and 0.26-21.28 mu g/ L, respectively. The cross-reactivities for the analogs of propiconazole were <1.9%. The average recoveries of the spiked samples were 75.7%-117.0% with relative standard deviations of <9.4%. The results of the ic-ELISA were consistent with those of gas chromatography-mass spectrometry in the analysis of blind samples. Therefore, we provide an alternative tool for the accurate and reliable determination of propiconazole.
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