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RNAPII Degradation Factor Def1 Is Required for Development, Stress Response, and Full Virulence of Magnaporthe oryzae

文献类型： 外文期刊

作者： Zhang, Xinrong 1 ; Li, Dong 1 ; Zhu, Jun 2 ; Zheng, Jing 2 ; Li, Hongye 2 ; He, Qixuan 1 ; Peng, Jun 3 ; Chen, Shen 4 ; Chen, Xiao-Lin 2 ; Wang, Weixiang 1 ;

作者机构： 1.Beijing Univ Agr, Coll Plant Sci & Technol, Natl Demonstrat Ctr Expt Plant Prod Educ, Beijing Key Lab New Technol Agr Applicat, Beijing 102206, Peoples R China

2.Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Prov Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Peoples R China

4.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

关键词： Magnaporthe oryzae; Def1; pathogenicity; O-GlcNAc; stress response; infection

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 4 期

页码：

收录情况： SCI

摘要： The RNA polymerase II degradation factor Degradation Factor 1 (Def1) is important for DNA damage repair and plays various roles in eukaryotes; however, the biological role in plant pathogenic fungi is still unknown. In this study, we investigated the role of Def1 during the development and infection of the rice blast fungus Magnaporthe oryzae. The deletion mutant of Def1 displayed slower mycelial growth, less conidial production, and abnormal conidial morphology. The appressoria of Delta def1 was impaired in the penetration into host cells, mainly due to blocking in the utilization of conidial storages, such as glycogen and lipid droplets. The invasive growth of the Delta def1 mutant was also retarded and accompanied with the accumulation of reactive oxygen species (ROS) inside the host cells. Furthermore, compared with the wild type, Delta def1 was more sensitive to multiple stresses, such as oxidative stress, high osmotic pressure, and alkaline/acidic pH. Interestingly, we found that Def1 was modified by O-GlcNAcylation at Ser232, which was required for the stability of Def1 and its function in pathogenicity. Taken together, the O-GlcNAc modified Def1 is required for hyphae growth, conidiation, pathogenicity, and stress response in M. oryzae. This study reveals a novel regulatory mechanism of O-GlcNAc-mediated Def1 in plant pathogenic fungi.

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