The development of genus-specific and species-specific real-time PCR assays for the authentication of Patagonian toothfish and Antarctic toothfish in commercial seafood products
文献类型： 外文期刊
作者： Yao, Lin 1 ; Qu, Meng 1 ; Jiang, Yanhua 1 ; Guo, Yingying 1 ; Li, Na 1 ; Li, Fengling 1 ; Tan, Zhijun 1 ; Wang, Lianzhu 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
关键词： Patagonian toothfish; Antarctic toothfish; authentication; real-time PCR
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.639； 五年影响因子：3.803 ）
ISSN： 0022-5142
年卷期：
页码：
收录情况： SCI
摘要： BACKGROUND The substitution or mislabeling of toothfish is an issue of significant concern for seafood authorities; it also reduces the effectiveness of marine conservation and management programs for its over-exploitation and illegal trafficking, boosting the need for identification methods. RESULTS Two species-specific real-time polymerase chain reaction (PCR) assays for the identification of Patagonian toothfish (Dissostichus eleginoides) and Antarctic toothfish (Dissostichus mawsoni) and a genus-specific real-time PCR assay for Dissostichus spp. identification were developed based on fragments of the 16S rRNA and COI (cytochrome c oxidase subunit I) genes. These methods were confirmed to be rapid, simple, and sensitive (absolute sensitivity of 0.0002 ng mu L-1 and relative sensitivity of 0.1 g kg(-1) with good specificity). These methods can be applied to processed and commercial fish products. CONCLUSIONS These approaches can be beneficial for protecting both consumers and producers from economic fraud and might also help protect toothfish from over-exploitation as well as combat illegal, unreported, and unregulated (IUU) fisheries. (c) 2021 Society of Chemical Industry.
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