文献类型： 外文期刊

作者： Li, Cheng ¹ ; Huang, Wenjie ² ; Han, Xiaoxu ¹ ; Zhao, Guohua ⁶ ; Zhang, Wenyang ² ; He, Weijun ¹ ; Nie, Bao ¹ ; Chen, Xufeng ² ; Zhang, Taijie ⁷ ; Bai, Wenhui ¹ ; Zhang, Xiaopeng ¹ ; He, Jingjing ¹ ; Zhao, Cheng ¹ ; Fernie, Alisdair R. ⁵ ; Tschaplinski, Timothy J. ³ ; Yang, Xiaohan ³ ; Yan, Shijuan ² ; Wang, Li ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab,Minist Agr, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr, Shenzhen, Peoples R China

2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou, Peoples R China

3.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

4.Ctr Bioenergy Innovat, Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

5.Max Planck Inst Mol Plant Physiol, Muhlenberg 1, D-14476 Potsdam, Germany

6.Shenzhen & Chinese Acad Sci, Key Lab Southern Subtrop Plant Divers, Fairy Lake Bot Garden, Shenzhen 518004, Peoples R China

7.Guangdong Acad Agr Sci, Key Lab Green Prevent & Control Fruits & Vegetable, Guangdong Prov Key Lab High Technol Plant Protect, Inst Plant Protect,Minist Agr & Rural Affairs, Guangzhou 510640, Peoples R China

8.Chinese Acad Agr Sci, Kunpeng Inst Modern Agr Foshan, Foshan, Peoples R China

关键词： Platycerium bifurcatum; crassulacean acid metabolism; multi-omics; transcription factor; PPCK; convergent evolution

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 5 期

页码：

收录情况： SCI

摘要： Crassulacean acid metabolism (CAM) has high water-use efficiency (WUE) and is widely recognized to have evolved from C3 photosynthesis. Different plant lineages have convergently evolved CAM, but the molec-ular mechanism that underlies C3-to-CAM evolution remains to be clarified. Platycerium bifurcatum (elk -horn fern) provides an opportunity to study the molecular changes underlying the transition from C3 to CAM photosynthesis because both modes of photosynthesis occur in this species, with sporotrophophyll leaves (SLs) and cover leaves (CLs) performing C3 and weak CAM photosynthesis, respectively. Here, we report that the physiological and biochemical attributes of CAM in weak CAM-performing CLs differed from those in strong CAM species. We investigated the diel dynamics of the metabolome, proteome, and transcriptome in these dimorphic leaves within the same genetic background and under identical environ-mental conditions. We found that multi-omic diel dynamics in P. bifurcatum exhibit both tissue and diel ef-fects. Our analysis revealed temporal rewiring of biochemistry relevant to the energy-producing pathway (TCA cycle), CAM pathway, and stomatal movement in CLs compared with SLs. We also confirmed that PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE (PPCK) exhibits convergence in gene expression among highly divergent CAM lineages. Gene regulatory network analysis identified candidate transcription factors regulating the CAM pathway and stomatal movement. Taken together, our results provide new in-sights into weak CAM photosynthesis and new avenues for CAM bioengineering.