您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa

文献类型： 外文期刊

作者： Zhang, Jiefu 2 ; Lu, Ying 1 ; Yuan, Yuxiang 3 ; Zhang, Xiaowei 3 ; Geng, Jianfeng 3 ; Chen, Yu 1 ; Cloutier, Sylvie 4 ; M 1 ;

作者机构： 1.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

3.Henan Acad Agr Sci, Zhengzhou 450001, Peoples R China

4.Agr Agri Food Canada, Cereal Res Ctr, Winnipeg, MB R3T 2N2, Canada

关键词： Brassica rapa;Seed coat color;Hairiness;Map-based cloning;TTG1 homolog

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A glabrous, yellow-seeded doubled haploid (DH) line and a hairy, black-seeded DH line in Chinese cabbage (B. rapa) were used as parents to develop a DH line population that segregated for both hairiness and seed coat color traits. The data showed that both traits completely co-segregated each other, suggesting that one Mendelian locus controlled both hairiness and seed coat color in this population. A fine genetic map was constructed and a SNP marker that was located inside a Brassica ortholog of TRANSPARENT TESTA GLABRA 1 (TTG1) in Arabidopsis showed complete linkage to both the hairiness and seed coat color gene, suggesting that the Brassica TTG1 ortholog shared the same gene function as its Arabidopsis counterpart. Further sequence analysis of the alleles from hairless, yellow-seeded and hairy, black-seeded DH lines in B. rapa showed that a 94-base deletion was found in the hairless, yellow-seeded DH lines. A nonfunctional truncated protein in the hairless, yellow-seeded DH lines in B. rapa was suggested by the coding sequence of the TTG1 ortholog. Both of the TTG1 homologs from the black and yellow seeded B. rapa lines were used to transform an Arabidopsis ttg1 mutant and the results showed that the TTG1 homolog from the black seeded B. rapa recovered the Arabidopsis ttg1 mutant, while the yellow seeded homolog did not, suggesting that the deletion in the Brassica TTG1 homolog had led to the yellow seeded natural mutant. This was the first identified gene in Brassica species that simultaneously controlled both hairiness and seed coat color traits.

  • 相关文献

[1]Map-Based Cloning of zb7 Encoding an IPP and DMAPP Synthase in the MEP Pathway of Maize. Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Wang, Zhen-Hua,Zhao, Yuan-Zeng. 2012

[2]Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa. Braynen, Janeen,Yang, Yan,Wei, Fang,Cao, Gangqiang,Shi, Gongyao,Tian, Baoming,Jia, Hao,Wei, Zhenzhen,Braynen, Janeen,Zhang, Xiaowei,Jia, Hao,Wei, Xiaochun,Wei, Zhenzhen. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台