Isolation and characterization of polymorphic microsatellite loci from bluefin leatherjacket (Navodon septentrionalis Gunther, 1877)
文献类型: 外文期刊
作者: Xu, Gen-Bo 1 ; Tian, Yong-Sheng 1 ; Liao, Xiao-Lin 1 ; Chen, Song-Lin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
关键词: Microsatellite;Bluefin leatherjacket;Navodon septentrionalis;Enriched genomic library
期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )
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收录情况: SCI
摘要: The first set of 18 polymorphic microsatellite markers were developed from bluefin leatherjacket (Navodon septentrionalis Gunther, 1877). From a (GT)(n)-enriched genomic library, we got 121 microsatellites, of which 60 were randomly selected for designing microsatellite primers. Eighteen of these loci were polymorphic in a test population of 32 individuals with alleles ranging from 2 to 9, and expected and observed heterozygosities from 0.1463 to 0.8517 and from 0.1562 to 1.0000, respectively. No significant linkage disequilibrium between pairs of loci was found, but three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate population structure in bluefin leatherjacket.
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