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Gene expression changes in response to low temperatures in embryos of the kelp grouper, Epinephelus moara

文献类型： 外文期刊

作者： Chen, Zhang-Fan 1 ; Tian, Yong-Sheng 1 ; Ma, Wen-Hui 3 ; Zhai, Jie-Ming 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Peoples R China

3.Ming Bo Aquat Co Ltd, Laizhou 261400, Peoples R China

关键词： Grouper (Epinephelus moara); Embryos; Cooling; RNA-Seq; Differential gene expression

期刊名称：CRYOBIOLOGY （ 影响因子：2.487； 五年影响因子：2.641 ）

ISSN： 0011-2240

年卷期： 2020 年 97 卷

页码：

收录情况： SCI

摘要： The kelp grouper Epinephelus moara has high economic value and is popular in fisheries and aquaculture in China. In the previous study, we treated the embryos at 16-22 somite stage at 4 degrees C, 25.7 degrees C, 140 degrees C and 196 degrees C, and successfully obtained surviving embryos in each group. To better understand the molecular changes affected by the low temperatures, we conducted a comparative transcriptome analysis among embryos exposed at 4 degrees C for 30 min, embryos exposed at 25.7 degrees C for 30 min and the control group. qPCR assays were conducted for the validation. Signal transduction pathways were highly enriched for the differentially expressed genes. c-Fos, c-Jun, JunD, GADD45, involved in MAPK signaling pathway, were upregulated when embryos were treated at low temperatures. As immediate early genes, Egr-1a and b, and IER2, that respond quickly to the environment stress, their expression increased as well. Hsp70 showed similar expression pattern as immediate early genes. Meanwhile, transcription factors Sox, HES, TFIID, muscle movement and protein synthesis-related genes were downregulated. Taken together, our findings suggest that cooling disrupts gene expression patterns in E. moara embryos. The differentially expressed genes may be involved in cellular resistance against low temperatures, possibly through neural activation, apoptosis, proliferation, differentiation, cellular recovery and heat shock regulation. This study also provides transcriptome dataset of E. moara embryos exposed to cold temperatures for future studies focusing on the molecular effects of cryopreservation.

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