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Isolation and characterization of polymorphic microsatellite loci from fat greenling (Hexagrammos otakii)

文献类型: 外文期刊

作者: Chen, Song-Lin 1 ; Miao, Gui-Dong 1 ; Shao, Chang-Wei 1 ; Tian, Yong-Sheng 1 ; Liao, Xiaolin 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

关键词: Fat greenling;Hexagrammos otakii;Enriched genomic library;SSR;Microsatellites

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN: 1566-0621

年卷期: 2009 年 10 卷 5 期

页码:

收录情况: SCI

摘要: Fat greenling (Hexagrammos otakii) is an economocally important marine fish species. Thirtyfive microsatellite loci were isolated from two enriched genomic library of Hexagrammos otakii. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and expected heterozygosities per locus from 0.2581 to 1.0000 and from 0.2892 to 0.7726, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Hexagrammos otakii.

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