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New polymorphic microsatellite markers for bluefin leatherjacket (Navodon septentrionalis Gunther, 1877)

文献类型: 外文期刊

作者: Xu, Gen-Bo 1 ; Chen, Song-Lin 1 ; Tian, Yong-Sheng 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

关键词: Bluefin leatherjacket;Enriched genomic library;Microsatellite;Navodon septentrionalis

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A microsatellite-enriched genomic DNA library of Navodon septentrionalis was generated and screened by sequencing. Ten dinucleotide microsatellite loci were characterized by genotyping 24 samples. The observed number of alleles ranged from two to seven with an average of 4. 40, while the effective number of alleles ranged from 1. 49 to 5. 70 with an average of 3. 31. The observed and expected heterozygosities ranged from 0. 2917 to 0. 9167 and from 0. 3369 to 0. 8422, respectively. No significant linkage disequilibrium between pairs of loci was found, but one loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci would be useful for investigating genetic population structure and molecule-assisted breeding for N. septentrionalis.

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