文献类型： 外文期刊

作者： Huang, Ke-Jing ¹ ; Wei, Cai-Yun ¹ ; Liu, Wei-Li ¹ ; Xie, Wan-Zhen ¹ ; Zhang, Jun-Feng ² ; Wang, Wei ³ ;

作者机构： 1.Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China

2.Henan Acad Agr Sci, Res Ctr Agr Stand & Inspect Technol, Chengzhou 450002, Peoples R China

3.Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224003, Peoples R China

关键词： Ultrasound-assisted dispersive liquid-liquid microextraction;Biogenic amines;2;6-Dimethyl-4-quinolinecarboxylic acid;N-hydroxysuccinimide ester;High-performance liquid chromatography-fluorescence detection;Rice wine samples

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

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收录情况： SCI

摘要： Ultrasound-assisted dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography-fluorescence detection was used for the extraction and determination of three biogenic amines including octopamine. tyramine and phenethylamine in rice wine samples. Fluorescence probe 2.6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester was applied for derivatization of biogenic amines. Acetonitrile and 1-octanol were used as disperser solvent and extraction solvent, respectively. Extraction conditions including the type of extraction solvent, the volume of extraction solvent, ultrasonication time and centrifuging time were optimized. After extraction and centrifuging, analyte was injected rapidly into high-performance liquid chromatography and then detected with fluorescence. The calibration graph of the proposed method was linear in the range of 5-500 mu g mL(-1) (octopamine and tyramine) and 0.025-2.5 mu g mL(-1) (phenethylamine). The relative standard deviations were 2.4-3.2% (n = 6) and the limits of detection were in the range of 0.02-5 ng mL(-1). The method was applied to analyze the rice wine samples and spiked recoveries in the range of 95.42-104.56% were obtained. The results showed that ultrasound-assisted dispersive liquid-liquid microextraction was a very simple, rapid, sensitive and efficient analytical method for the determination of trace amount of biogenic amines.