文献类型： 外文期刊

作者： Huang, Ke-Jing ¹ ; Wei, Cai-Yun ¹ ; Xie, Wan-Zhen ¹ ; Liu, Yan-Ming ¹ ; Chen, Yong-Hong ² ; Zhang, Jun-Feng ³ ;

作者机构： 1.Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China

2.Xinyang Cent Hosp, Xinyang 464000, Peoples R China

3.Henan Acad Agr Sci, Res Ctr Agr Stand & Inspect Technol, Zhengzhou 450002, Peoples R China

关键词： Column liquid chromatography;Fluorescence detection;Aliphatic primary;amines and diamines;2;6-Dimethyl-4-quinolinecarboxylic acid;N-hydroxysuccinimide ester

期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）

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收录情况： SCI

摘要： A method for sensitive simultaneous analysis of aliphatic primary amines and diamines has been developed and validated. The compounds were analyzed by reversed-phase high-performance liquid chromatography after pre-column derivatization with 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester as fluorescent probe. The derivatization reaction was performed at 50 A degrees C for 40 min in 0.1 mol L-1 borax buffer solution (pH 7.5). The resulting fluorophores were separated to baseline on a C-18 column and fluorimetrically detected at lambda (ex)/lambda (em) = 326/409 nm. Detection limits were in the range 0.50-0.02 nmol L-1. The method was successfully used for analysis of aliphatic amines in water, human urine, and serum.