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The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner

文献类型： 外文期刊

作者： Li, Kaihuai 1 ; Xu, Gaoge 1 ; Wang, Bo 1 ; Wu, Guichun 1 ; Hou, Rongxian 1 ; Liu, Fengquan 1 ;

作者机构： 1.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety State Key Lab, Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

期刊名称：COMMUNICATIONS BIOLOGY （ 影响因子：6.268； 五年影响因子：6.268 ）

ISSN：

年卷期： 2021 年 4 卷 1 期

页码：

收录情况： SCI

摘要： Soil bacteria often harbour various toxins to against eukaryotic or prokaryotic. Diffusible signal factors (DSFs) represent a unique group of quorum sensing (QS) chemicals that modulate interspecies competition in bacteria that do not produce antibiotic-like molecules. However, the molecular mechanism by which DSF-mediated QS systems regulate antibiotic production for interspecies competition remains largely unknown in soil biocontrol bacteria. In this study, we find that the necessary QS system component protein RpfG from Lysobacter, in addition to being a cyclic dimeric GMP (c-di-GMP) phosphodiesterase (PDE), regulates the biosynthesis of an antifungal factor (heat-stable antifungal factor, HSAF), which does not appear to depend on the enzymatic activity. Interestingly, we show that RpfG interacts with three hybrid two-component system (HyTCS) proteins, HtsH1, HtsH2, and HtsH3, to regulate HSAF production in Lysobacter. In vitro studies show that each of these proteins interacted with RpfG, which reduced the PDE activity of RpfG. Finally, we show that the cytoplasmic proportions of these proteins depended on their phosphorylation activity and binding to the promoter controlling the genes implicated in HSAF synthesis. These findings reveal a previously uncharacterized mechanism of DSF signalling in antibiotic production in soil bacteria. Li et al shows that the quorum sensing system component protein RpfG from Lysobacter, in addition to being a cyclic dimeric GMP (c-di-GMP) phosphodiesterase, also regulates the biosynthesis of an antifungal factor. They show that RpfG regulates the production of HSAF through a direct interaction with three hybrid two component system (HyTCS) proteins, providing insights into the antifungal defence in soil bacteria.

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