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Proteomic characterization of the different stages of seed germination in Cupressus gigantea

文献类型： 外文期刊

作者： Li, Jianxin 1 ; Zhang, Subin 3 ; Lei, Pei 1 ; Eh, Tong-Ju 3 ; Zhang, Yu 4 ; Jin, Guangze 5 ; Dong, Kun 4 ; Xu, Qijiang 8 ; Luo, Qiuxiang 3 ; Meng, Fanjuan 1 ;

作者机构： 1.Jilin Agr Univ, Coll Forestry & Grasslands, Changchun 130118, Peoples R China

2.Jilin Prov Key Lab Tree & Grass Genet & Breeding, Changchun 130118, Peoples R China

3.Northeast Forestry Univ, Coll Life Sci, Harbin, Peoples R China

4.Heilongjiang Acad Agr Sci, Hort Sub Acad, Harbin 150069, Peoples R China

5.Northeast Forestry Univ, Ctr Ecol Res, Harbin 150040, Peoples R China

6.Northeast Forestry Univ, Key Lab Sustainable Forest Ecosyst Management, Minist Educ, Harbin 150040, Peoples R China

7.Northeast Forestry Univ, Northeast Asia Biodivers Res Ctr, Harbin 150040, Peoples R China

8.Youjiang Med Univ Nationalities, Sch Lab Med, Baise, Peoples R China

关键词： Cupressus gigantea; proteomics; seed germination; two-dimensional electrophoresis (2-DE)

期刊名称：SEED SCIENCE RESEARCH （ 影响因子：2.1； 五年影响因子：2.5 ）

ISSN： 0960-2585

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Seed germination is a pivotal period of plant growth and development. This process can be divided into four major stages, swelling absorption, seed coat dehiscence, radicle emergence and radicle elongation. Cupressus gigantea, a tree native to Tibet, China, is characterized by its resistance to stresses such as cold, and drought, and has a high economic and ecological value. Nevertheless, given its unique geographic location, its seeds are difficult to germinate. Therefore, it is crucial to explore the mechanisms involved in seed germination in this species to improve the germination efficiency of its seeds, thereby protecting this high-quality resource. Here, our findings indicate that seed germination was enhanced when exposed to a 6-h/8-h light/dark photoperiod, coupled with a temperature of 20 degrees C. Furthermore, the application of exogenous GA(3) (1 mg/ml, about 2.9 mM) stimulated the germination of C. gigantea seeds. Subsequently, proteomics was used to detect changes in protein expression during the four stages of seed germination. We identified 34 differentially expressed proteins (DEPs), including 13 at the radicle pre-emergence stage, and 17 at the radicle elongation stage. These DEPs were classified into eight functional groups, cytoskeletal proteins, energy metabolism, membrane transport, stress response, molecular chaperones, amino acid metabolism, antioxidant system and ABA signalling pathway. Most of them were found to be closely associated with amino acid metabolism. Combined, these findings indicate that, along with temperature and light, exogenous GA(3) can increase the germination efficiency of C. gigantea seeds. Our study also offers insights into the changes in protein expression patterns in C. gigantea seeds during germination.

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