文献类型： 外文期刊

作者： Yu, Min ¹ ; Xiong, Jinyu ² ; Dong, Kun ³ ; Quan, Xin ² ; Guo, Hao ² ; Huo, Junwei ² ; Qin, Dong ² ; Wang, Yanchang ⁵ ; Lu, Xuemei ⁵ ; Zhu, Chenqiao ² ;

作者机构： 1.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

2.Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China

3.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150040, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops Northe, Harbin 150030, Peoples R China

5.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty Ag, Wuhan Bot Garden, Wuhan 430074, Peoples R China

关键词： Actinidia chinensis; transcription factor; fruit coloration; MYBs

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Light and temperature are key factors influencing the accumulation of anthocyanin in fruit crops. To assess the effects of fruit bagging during development and high post-ripening temperature on 'Hongyang' kiwifruit, we compared the pigmentation phenotypes and expression levels of anthocyanin-related genes between bagged and unbagged treatments, and between 25 degrees C and 37 degrees C postharvest storage temperatures. Both the bagging and 25 degrees C treatments showed better pigmentation phenotypes with higher anthocyanin concentrations. The results of the qRT-PCR analysis revealed that the gene expression levels of LDOX (leucoanthocyanidin dioxygenase), F3GT (UDP-flavonoid 3-O-glycosyltransferase ), AcMYB10, and AcbHLH42 were strongly correlated and upregulated by both the bagging treatment and 25 degrees C storage. The results of bimolecular fluorescence complementation and luciferase complementation imaging assays indicated an interaction between AcMYB10 and AcbHLH42 in plant cells, whereas the results of a yeast one-hybrid assay further demonstrated that AcMYB10 activated the promoters of AcLODX and AcF3GT. These results strongly suggest that enhanced anthocyanin synthesis is caused by the promoted expression of AcLODX and AcF3GT, regulated by the complex formed by AcMYB10-AcbHLH42.