Multi-omics analyses and botanical perfumer hypothesis provide insights into the formation and maintenance of aromatic characteristics of Dendrobium loddigesii flowers
文献类型： 外文期刊
作者： Du, Zhihui 1 ; Jin, Yuxuan 1 ; Yang, Xiyu 1 ; Xia, Kuaifei 2 ; Chen, Zhilin 1 ;
作者机构： 1.Guizhou Acad Agr Sci, Guizhou Hort Inst, Hort Engn Technol Res Ctr Guizhou, Guiyang 550000, Peoples R China
2.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China
关键词： Dendrobium loddigesii; Multi-omics; Terpenoids; Aromatic characteristics; Botanical perfumer hypothesis
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.1； 五年影响因子：6.2 ）
ISSN： 0981-9428
年卷期： 2024 年 214 卷
页码：
收录情况： SCI
摘要： Dendrobium loddigesii, a member of the Orchidaceae family, is a valuable horticultural crop known for its aromatic qualities. However, the mechanisms responsible for the development of its aromatic characteristics remain poorly understood. To elucidate these underlying mechanisms, we assembled the first chromosome-level reference genome of D. loddigesii using PacBio HiFi-reads, Illumina short-reads, and Hi -C data. The assembly comprises 19 pseudochromosomes with N50 contig and N50 scaffold sizes of 55.15 and 89.94 Mb, respectively, estimating the genome size to be 1.68 Gb, larger than that of other sequenced Dendrobium species. During the flowering stages, we conducted a comprehensive analysis combining volatilomics and transcriptomics to understand the characteristics and biosynthetic mechanisms pathways of the floral scent. Our findings emphasize the significant contribution of aromatic terpenoids, especially monoterpenoids, in defining the floral aroma. Furthermore, we identified two crucial terpene synthase (TPS) genes that play a key role in maintaining the aroma during flowering. Through the integration volatilomics data with catalytic assays of DlTPSbs proteins, we identified specific compounds responsible for the aromatic characteristics of D. loddigesii . This integrated analysis of the genome, transcriptome, and volatilome, offers valuable insights into the development and preservation of D. loddigesii 's aromatic characteristics, setting the stage for further exploration of the botanical perfumer hypothesis.
- 相关文献
作者其他论文 更多>>
-
Two new species of Begoniaceae from China mainland
作者：Tian, Daike;Wang, Songwei;Li, Xin;Tian, Daike;Wei, Hongjing;Wang, Songwei;Li, Xin;Huang, Cunzhong;Wu, Lei;Chen, Zhilin;Guo, Zhiyou;Gao, Qingmei;Sun, Xiaojun
关键词：WORDS; Begonia ruchengensis; Begonia auritalata; new taxa; taxonomy; flora of China; morphology; conservation
-
Integrative Analysis of Transcriptome and Metabolome Sheds Light on Flavonoid Biosynthesis in the Fruiting Body of Stropharia rugosoannulata
作者：Wu, Xian;Fu, Shaobin;Du, Zhihui;Liu, Lian;Chen, Zhilin;Li, Yurong
关键词：flavonoids; Stropharia rugosoannulata; fruiting body; metabolomics; transcriptomics
-
Aromatic Terpenes and Their Biosynthesis in Dendrobium, and Conjecture on the Botanical Perfumer Mechanism
作者：Du, Zhihui;Yang, Xiyu;Jin, Yuxuan;Wang, Weize;Chen, Zhilin;Zhou, Shuting;Xia, Kuaifei
关键词：Dendrobium; floral scent; aromatic characteristics; terpene synthase; botanical perfumer mechanism
-
Multi-Omics Analyses Unravel Metabolic and Transcriptional Differences in Tender Shoots from Two Sechium edule Varieties
作者：Du, Zhihui;Qu, Fei;Zhang, Chaojun;Chen, Zhilin;Li, Yurong;Wen, Linhong
关键词：Sechium edule; chaylte vines; flavonoids; metabolomics; transcriptomics
-
An Integrated Analysis of Metabolomics and Transcriptomics Reveals Significant Differences in Floral Scents and Related Gene Expression between Two Varieties of Dendrobium loddigesii
作者：Wang, Sha;Du, Zhihui;Yang, Xiyu;Wang, Lanlan;Chen, Zhilin;Xia, Kuaifei
关键词：Dendrobium loddigesii; volatile metabolites; floral scent; transcriptomics; GC-MS
-
Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum
作者：Du, Zhihui;Jin, Yuxuan;Wang, Weize;Chen, Zhilin;Xia, Kuaifei;Xia, Kuaifei
关键词：Dendrobium chrysotoxum; floral scent; widely-targeted volatilomics; terpenes; RNA-Seq
-
The complete chloroplast genome of Cymbidium dayanum (Orchidaceae)
作者：Du, Zhihui;Yang, Xiyu;Tan, Guofei;Chen, Zhilin
关键词：Cymbidium dayanum; chloroplast genome; phylogenetic analysis
