Deciphering the Crucial Roles of the Quorum-Sensing Transcription Factor SdiA in NADPH Metabolism and (S)-Equol Production in Escherichia coli Nissle 1917
文献类型： 外文期刊
作者： Wang, Zhe 1 ; Dai, Yiqiang 1 ; Azi, Fidelis 3 ; Dong, Mingsheng 1 ; Xia, Xiudong 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
3.Guangdong Technion Israel Inst Technol, Dept Chem Engn, Shantou 515063, Peoples R China
4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
5.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
关键词： isoflavone; (S)-equol; quorum sensing; E. coli Nissle 1917; NADPH; biosynthesis
期刊名称：ANTIOXIDANTS （ 影响因子：7.0； 五年影响因子：7.3 ）
ISSN：
年卷期： 2024 年 13 卷 3 期
页码：
收录情况： SCI
摘要： The active metabolite (S)-equol, derived from daidzein by gut microbiota, exhibits superior antioxidative activity compared with its precursor and plays a vital role in human health. As only 25% to 50% of individuals can naturally produce equol when supplied with isoflavone, we engineered probiotic E. coli Nissle 1917 (EcN) to convert dietary isoflavones into (S)-equol, thus offering a strategy to mimic the gut phenotype of natural (S)-equol producers. However, co-fermentation of EcN-eq with fecal bacteria revealed that gut microbial metabolites decreased NADPH levels, hindering (S)-equol production. Transcriptome analysis showed that the quorum-sensing (QS) transcription factor SdiA negatively regulates NADPH levels and (S)-equol biosynthesis in EcN-eq. Screening AHLs showed that SdiA binding to C10-HSL negatively regulates the pentose phosphate pathway, reducing intracellular NADPH levels in EcN-eq. Molecular docking and dynamics simulations investigated the structural disparities in complexes formed by C10-HSL with SdiA from EcN or E. coli K12. Substituting sdiA_EcN in EcN-eq with sdiA_K12 increased the intracellular NADPH/NADP+ ratio, enhancing (S)-equol production by 47%. These findings elucidate the impact of AHL-QS in the gut microbiota on EcN NADPH metabolism, offering insights for developing (S)-equol-producing EcN probiotics tailored to the gut environment.
- 相关文献
作者其他论文 更多>>
-
Inhibition of Fermented and Unfermented Soy Whey Extracts on the Quorum Sensing and Biogenic Amine Production of Morganella morganii wf-1
作者：Dai, Yiqiang;Zhong, Jiajing;Geng, Doudou;Dong, Jiajia;Dai, Yiqiang;Yin, Liqing;Xia, Xiudong;Yin, Liqing;Xia, Xiudong
关键词：Biogenic amines; Morganella morganii; Quorum sensing; Unfermented soy whey extract; Fermented soy whey extract
-
Preparation, Characterization and Stability of Calcium-Binding Peptides Derived from Chicken Blood
作者：Yang, Jing;Wang, Daoying;Yang, Jing;Shi, Jing;Zou, Ye;Xu, Weimin;Xia, Xiudong;Wang, Daoying;Zhou, Ying
关键词：chicken hemoglobin peptides; calcium-binding capacity; characterization; stability
-
Developing Ganoderma lucidum as a next-generation cell factory for food and nutraceuticals
作者：Azi, Fidelis;Chen, Wenhao;Lin, Dewei;Xu, Peng;Azi, Fidelis;Chen, Wenhao;Lin, Dewei;Xu, Peng;Wang, Zhe;Xu, Peng
关键词：
-
Constructing Protein-Scaffolded Multienzyme Assembly Enhances the Coupling Efficiency of the P450 System for Efficient Daidzein Biosynthesis from (2S)-Naringenin
作者：Wang, Zhe;Dai, Yiqiang;Dong, Mingsheng;Xia, Xiudong;Wang, Zhe;Dai, Yiqiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Azi, Fidelis;Wang, Zhongjiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Xia, Xiudong
关键词：scaffold multienzyme system; daidzein; coupling efficiency; ROS
-
Effects of water-soluble and water-insoluble α-glucans produced in situ by Leuconostoc citreum SH12 on physicochemical properties of fermented soymilk and their structural analysis
作者：Dai, Yiqiang;Ge, Zhiwen;Wang, Zhe;Xu, Weimin;Wang, Daoying;Dong, Mingsheng;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang
关键词：Fermented soymilk; Leuconostoc citreum SH12; Water-soluble alpha-glucan; Water -insoluble alpha-glucan; Physicochemical properties
-
Microbiome-metabolomics-based insight into the protective effects of dietary fiber from sweetpotato residues on the high-fat diet-induced intestinal integrity damage
作者：Yin, Liqing;Jin, Yunyi;Lu, Xinghua;Cheng, Jiyu;Guan, Yuge;Cheng, Junfeng;Lu, Guoquan;Pang, Linjiang;Azi, Fidelis;Xia, Xiudong
关键词：Dietary fiber; Physicochemical property; Metabolomics analysis
-
Engineering Escherichia coli for cost-effective production of medium-chain fatty acids from soy whey using an optimized galactose-based autoinduction system
作者：Wang, Zhe;Dai, Yiqiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Wang, Zhe;Dai, Yiqiang;Dong, Mingsheng;Xia, Xiudong;Azi, Fidelis;Wang, Zhongjiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Xia, Xiudong
关键词：Medium-chain fatty acids; Soy whey; Autoinduction system; Escherichia coli; Metabolic engineering
