Deciphering the Crucial Roles of the Quorum-Sensing Transcription Factor SdiA in NADPH Metabolism and (S)-Equol Production in Escherichia coli Nissle 1917
文献类型： 外文期刊
作者： Wang, Zhe 1 ; Dai, Yiqiang 1 ; Azi, Fidelis 3 ; Dong, Mingsheng 1 ; Xia, Xiudong 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
3.Guangdong Technion Israel Inst Technol, Dept Chem Engn, Shantou 515063, Peoples R China
4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
5.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
关键词： isoflavone; (S)-equol; quorum sensing; E. coli Nissle 1917; NADPH; biosynthesis
期刊名称：ANTIOXIDANTS （ 影响因子：7.0； 五年影响因子：7.3 ）
ISSN：
年卷期： 2024 年 13 卷 3 期
页码：
收录情况： SCI
摘要： The active metabolite (S)-equol, derived from daidzein by gut microbiota, exhibits superior antioxidative activity compared with its precursor and plays a vital role in human health. As only 25% to 50% of individuals can naturally produce equol when supplied with isoflavone, we engineered probiotic E. coli Nissle 1917 (EcN) to convert dietary isoflavones into (S)-equol, thus offering a strategy to mimic the gut phenotype of natural (S)-equol producers. However, co-fermentation of EcN-eq with fecal bacteria revealed that gut microbial metabolites decreased NADPH levels, hindering (S)-equol production. Transcriptome analysis showed that the quorum-sensing (QS) transcription factor SdiA negatively regulates NADPH levels and (S)-equol biosynthesis in EcN-eq. Screening AHLs showed that SdiA binding to C10-HSL negatively regulates the pentose phosphate pathway, reducing intracellular NADPH levels in EcN-eq. Molecular docking and dynamics simulations investigated the structural disparities in complexes formed by C10-HSL with SdiA from EcN or E. coli K12. Substituting sdiA_EcN in EcN-eq with sdiA_K12 increased the intracellular NADPH/NADP+ ratio, enhancing (S)-equol production by 47%. These findings elucidate the impact of AHL-QS in the gut microbiota on EcN NADPH metabolism, offering insights for developing (S)-equol-producing EcN probiotics tailored to the gut environment.
- 相关文献
作者其他论文 更多>>
-
The use of dextran in 3D printing for dysphagia foods: Relationships between its structure and physicochemical properties
作者：Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Xia, Xiudong;Wang, Daoying;Dai, Yiqiang;Liu, Yifei;Wang, Zhe;Xu, Weimin;Dong, Mingsheng;Xia, Xiudong;Wang, Daoying;Xu, Weimin;Xia, Xiudong;Wang, Daoying
关键词：Dextran; Structure; Physicochemical properties; 3D printing whole grain and legume food; Dysphagia
-
Ingenious integration of synthetic biology and droplet microfluidics
作者：Zhang, Panrui;Wu, Haoyu;Zhang, Runxin;Zhao, Danshan;Wei, Wei;Yang, Qiaoyi;Shi, Tianqiong;Wang, Yuetong;Zhang, Panrui;Wu, Haoyu;Zhang, Runxin;Zhao, Danshan;Wei, Wei;Yang, Qiaoyi;Shi, Tianqiong;Wang, Yuetong;Wang, Zhe;Wang, Zhe
关键词：Synthetic biology; Droplet microfluidics; High-throughput; Green biofabrication; Cell-free systems
-
Advanced droplet microfluidic platform for high-throughput screening of industrial fungi
作者：Yang, Qiaoyi;Ye, Chao;Shi, Tianqiong;Wang, Yuetong;Yang, Qiaoyi;Lu, Siqi;Wu, Haoyu;Zhao, Danshan;Wei, Wei;Yi, Haoran;Li, Xi;Ye, Chao;Shi, Tianqiong;Wang, Yuetong;Ye, Chao;Wang, Zhe;Wang, Zhe
关键词：Droplet microfluidics; High-throughput screening; Industrial fungi; Filamentous fungi
-
3D printing of mycelium-enhanced plant-based protein composites for customizable texture in meat analogues
作者：Liu, Yifei;Dai, Yiqiang;Xia, Xiudong;Zhai, Kang;Jin, Yixia;Zhai, Yanfen;Dong, Mingsheng;Dai, Yiqiang;Xia, Xiudong;Zhao, Baomin
关键词：3D printing; Mycelium; Meat analogue; Fermentation; Texture; Porosity
-
Stabilizing effect of Leuconostoc mesenteroides Lm10 produced dextran in situ on stirred soy yogurt: Structure-function relationship
作者：Dai, Yiqiang;Wang, Zhe;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Dong, Mingsheng;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang;Wang, Daoying;Xia, Xiudong
关键词：Stirred soy yogurt; Leuconostoc mesenteroides Lm10; Dextran; Dextransucrase; Stability
-
Effect of low-frequency ultrasound pretreatment on taste substances in chicken liver by fermentation
作者：Wang, Xiaowen;Lan, Yibo;Yang, Biao;Yang, Jing;Ma, Jingjing;Cheng, Mei;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye;Wang, Xiaowen;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye;Wang, Xiaowen;Lan, Yibo;Yang, Biao;Yang, Jing;Ma, Jingjing;Cheng, Mei;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye
关键词：Chicken liver; Fermentation; Ultrasound pretreatment; Taste; Acid soluble protein
-
Enhanced mycoprotein production of Neurospora intermedia from soy processing byproducts: Integration of ultrasonic stimulation with repeated-batch simultaneous saccharification and culture
作者：Dai, Yiqiang;Wang, Zhe;Han, Siyu;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Liu, Yifei;Tao, Yang;Dong, Mingsheng;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang
关键词：Neurospora intermedia mycoprotein; Soy whey; Okara; Ultrasound treatment; Repeated-batch culture
