文献类型： 外文期刊
作者： Zhang, Panrui 1 ; Wu, Haoyu 1 ; Zhang, Runxin 1 ; Zhao, Danshan 1 ; Wei, Wei 1 ; Yang, Qiaoyi 1 ; Wang, Zhe 3 ; Shi, Tianqiong 1 ; Wang, Yuetong 1 ;
作者机构： 1.Nanjing Normal Univ, State Key Lab Microbial Technol, Nanjing 210023, Peoples R China
2.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210046, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
关键词： Synthetic biology; Droplet microfluidics; High-throughput; Green biofabrication; Cell-free systems
期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）
ISSN： 1385-8947
年卷期： 2025 年 520 卷
页码：
收录情况： SCI
摘要： As an interdisciplinary subject integrating biology, engineering and computer science, synthetic biology is committed to designing functional biological systems through engineering means to meet challenges in biomanufacturing, medicine and energy. However, traditional gene modification technology faces bottlenecks such as low efficiency of high-throughput screening, high reagent consumption and detection complexity. Droplet microfluidics constructs independent reaction units by precisely manipulating micron-sized droplets. With the advantages of compartmentalization, high throughput and low cost, it provides an innovative technology platform for synthetic biology. This article systematically reviews three major applications of this technology in synthetic biology: in green biomanufacturing, high-throughput detection of enzymes and other metabolites along with efficient strain selection can be achieved by encapsulating mutation libraries; in the field of biomedicine, combining single-cell sequencing and digital PCR technology can realize cell heterogeneity analysis and precise quantification of trace nucleic acids, and promote the development of organoids drug screening and microbial community analysis; in in vitro cell-free systems, key biological processes such as DNA synthesis, plasmid transformation and protein expression can be reconstructed through modular design within droplets. In addition, this review also discusses current technical challenges in chip design standardization, real-time detection with multi-parameters and large-scale production, as well as envisioning its potential directions in platform construction and industrial transformation for synthetic biology. The deep integration of droplet microfluidics will accelerate the transition of synthetic biology from basic research to practical application.
- 相关文献
作者其他论文 更多>>
-
The use of dextran in 3D printing for dysphagia foods: Relationships between its structure and physicochemical properties
作者：Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Xia, Xiudong;Wang, Daoying;Dai, Yiqiang;Liu, Yifei;Wang, Zhe;Xu, Weimin;Dong, Mingsheng;Xia, Xiudong;Wang, Daoying;Xu, Weimin;Xia, Xiudong;Wang, Daoying
关键词：Dextran; Structure; Physicochemical properties; 3D printing whole grain and legume food; Dysphagia
-
Advanced droplet microfluidic platform for high-throughput screening of industrial fungi
作者：Yang, Qiaoyi;Ye, Chao;Shi, Tianqiong;Wang, Yuetong;Yang, Qiaoyi;Lu, Siqi;Wu, Haoyu;Zhao, Danshan;Wei, Wei;Yi, Haoran;Li, Xi;Ye, Chao;Shi, Tianqiong;Wang, Yuetong;Ye, Chao;Wang, Zhe;Wang, Zhe
关键词：Droplet microfluidics; High-throughput screening; Industrial fungi; Filamentous fungi
-
Stabilizing effect of Leuconostoc mesenteroides Lm10 produced dextran in situ on stirred soy yogurt: Structure-function relationship
作者：Dai, Yiqiang;Wang, Zhe;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Dong, Mingsheng;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang;Wang, Daoying;Xia, Xiudong
关键词：Stirred soy yogurt; Leuconostoc mesenteroides Lm10; Dextran; Dextransucrase; Stability
-
Enhanced mycoprotein production of Neurospora intermedia from soy processing byproducts: Integration of ultrasonic stimulation with repeated-batch simultaneous saccharification and culture
作者：Dai, Yiqiang;Wang, Zhe;Han, Siyu;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Liu, Yifei;Tao, Yang;Dong, Mingsheng;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang
关键词：Neurospora intermedia mycoprotein; Soy whey; Okara; Ultrasound treatment; Repeated-batch culture
-
Constructing Protein-Scaffolded Multienzyme Assembly Enhances the Coupling Efficiency of the P450 System for Efficient Daidzein Biosynthesis from (2S)-Naringenin
作者：Wang, Zhe;Dai, Yiqiang;Dong, Mingsheng;Xia, Xiudong;Wang, Zhe;Dai, Yiqiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Azi, Fidelis;Wang, Zhongjiang;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Xia, Xiudong
关键词：scaffold multienzyme system; daidzein; coupling efficiency; ROS
-
Deciphering the Crucial Roles of the Quorum-Sensing Transcription Factor SdiA in NADPH Metabolism and (S)-Equol Production in Escherichia coli Nissle 1917
作者：Wang, Zhe;Dai, Yiqiang;Dong, Mingsheng;Xia, Xiudong;Wang, Zhe;Dai, Yiqiang;Xia, Xiudong;Azi, Fidelis;Xia, Xiudong;Xia, Xiudong
关键词：isoflavone; (S)-equol; quorum sensing; E. coli Nissle 1917; NADPH; biosynthesis
-
Effects of water-soluble and water-insoluble α-glucans produced in situ by Leuconostoc citreum SH12 on physicochemical properties of fermented soymilk and their structural analysis
作者：Dai, Yiqiang;Ge, Zhiwen;Wang, Zhe;Xu, Weimin;Wang, Daoying;Dong, Mingsheng;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Wang, Zhe;Xu, Weimin;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang
关键词：Fermented soymilk; Leuconostoc citreum SH12; Water-soluble alpha-glucan; Water -insoluble alpha-glucan; Physicochemical properties
