文献类型： 外文期刊

作者： Xu, Saiqing ¹ ; Ge, Shuai ¹ ; Xie, Ying ¹ ; Cheng, Jing ³ ; Ding, Ke ¹ ; Li, Huan ² ; Huang, Hua ⁴ ; Luo, Zisheng ⁶ ; Shan, Yang ¹ ; Ding, Shenghua ¹ ;

作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China

2.Hunan Acad Agr Sci, DongTing Lab, Hunan Agr Prod Proc Inst, Changsha 410125, Peoples R China

3.Hunan Key Lab Food Safety Sci Technol, Technol Ctr Changsha Customs, Changsha 410004, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China

5.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou, Peoples R China

6.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310000, Peoples R China

关键词： Ferulic acid ester derivatives; Water instability; Nanoemulsions; A. alternata; Fungicidal activity; Post-harvest storage

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.4； 五年影响因子：6.9 ）

ISSN： 0925-5214

年卷期： 2024 年 216 卷

页码：

收录情况： SCI

摘要： Postharvest fruit infection by Alternaria alternata results in significant food waste and economic losses. Methyl ferulate (MF) emerges as a safe and potent plant -derived agent against A. alternata , yet its effectiveness is hindered by poor water solubility, limiting its wide application in postharvest preservation. This study aimed to address this issue by utilizing naturally derived grapefruit essential oil (GEO) as the dissolution carrier for MF, followed by the preparation of a nanosystem to stabilize it through ultrasound -assisted methods. At 20 mg/mL MF loading, nanoemulsions (NEs) exhibited the smallest droplet size (15.66 nm), highest centrifugation stability (Ke of 2.32 %), and optimal storage stability (15.44 nm after 30 d at 4 degree celsius). Importantly, MF in the nanosystem (named MN) acts as an Ostwald ripening inhibitor and reduces the interfacial tension, which in turn benefits the stability of this system. In vitro , MN showed enhanced inhibition of A. alternata hyphal growth (an inhibitory rate in potato dextrose broth medium of 99.07 +/- 1.32 %) and a fourfold reduction in MIC compared to free MF. MN damaged A. alternata plasma membranes and induced reactive oxygen species (ROS) oxidative stress. In vivo , MN inhibited black spot disease and toxin production of A. alternata in pears. Additionally, MN incorporated into carboxymethylcellulose sodium (CMC) prolonged the shelf life of Korla pears and cherry tomatoes by delaying weight loss and maintaining their firmness and intrinsic nutrients. Therefore, this study broadened the application of MF, offering a viable approach for postharvest preservation of fruit infected by A. alternata .