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Development of broad-spectrum immunoassay with monoclonal antibody to detect five eugenols and study of their molecular recognition mechanism

文献类型： 外文期刊

作者： Luo, Lin 1 ; Kang, Rui-Yao 1 ; He, Zhen-Xi 1 ; Jia, Bao-Zhu 5 ; Chen, Zi-Jian 1 ; Deng, Hao 2 ; Xu, Zhen-Lin 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

2.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold chain Hainan P, Haikou 570100, Peoples R China

3.Heyuan Branch, Guangdong Lab Lingnan Modern Agr, Heyuan 517000, Peoples R China

4.Zhaoqing Univ, Sch Food & Pharmaceut Engn, Zhaoqing 526061, Peoples R China

5.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Peoples R China

关键词： Immunoassay; Hapten; Monoclonal antibody; Eugenol; Fish anesthetic

期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.1； 五年影响因子：6.4 ）

ISSN： 2590-1575

年卷期： 2024 年 22 卷

页码：

收录情况： SCI

摘要： In this study, three eugenol fragment-containing haptens were synthesized, and a monoclonal antibody (mAb) selective for five commonly-found eugenol compounds (EUGs, i.e., eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, and acetyl isoeugenol) was obtained. Based on this mAb, a broad-spectrum indirect competitive ELISA for high-throughput detection of five EUGs was developed. The detection limits for eugenol, isoeugenol, methyl eugenol, methyl isoeugenol and acetyl isoeugenol in both tilapia and shrimp samples were 25.3/ 50.6 mu g/ kg, 0.075/0.15 mu g/kg, 0.48/0.96 mu g/kg, 0.16/0.32 mu g/kg, and 18.16/36.32 mu g/kg, respectively. The recoveries for five EUGs ranged from 80.4 to 114.0 % with a coefficient of variation less than 11.5 %. Moreover, homology modelling and molecular docking were conducted to elucidate the interactions mechanism of mAb-EUGs. The work provides a promising tool for high-throughput screening of EUGs in aquatic products, which can serve as a benchmark for designing haptens and developing immunoassays for other small molecules.

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