Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
文献类型： 外文期刊
作者： Wu, Wenjin 1 ; Yang, Yuping 1 ; Wang, Lan 1 ; Xu, Tingting 2 ; Wang, Rui 3 ;
作者机构： 1.Hubei Acad Agr Sci, Farm Prod Proc Res Sub, Inst Agr Prod Proc & Nucl Agr Technol, Ctr Agr Sci & Technol,Ctr Hubei Innovat, Wuhan 430064, Peoples R China
2.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat F, Nanjing 210037, Peoples R China
3.Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Food Sci & Light Ind, Nanjing 211816, Peoples R China
4.Wuhan Inst Drug & Med Device Control, Wuhan 430075, Hubei, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）
ISSN：
年卷期： 2021 年 11 卷 30 期
页码：
收录情况： SCI
摘要： Staphylococcus aureus (S. aureus) is one of the most commonly isolated foodborne pathogens, and is considered as a major cause of foodborne illnesses worldwide. However, the development of smart and accurate analytical methods for the simultaneous detection and elimination of S. aureus in matrices of food or drinks remains challenging. In the present work, a mussel-inspired material, epsilon-poly-l-lysine-3,4-dihydroxy benzaldehyde (EPD), was designed and fabricated based on its Schiff base structure. Owing to the robust ability of the material to adhere onto wet electrode surfaces and the pH-responsive properties of EPD, the prepared immunosensor exhibited an excellent detection limit and linear range with on-demand antibacterial activity. In real milk samples, the average values obtained from the immunosensor were approximate to the standard results obtained from the plate count method, and the relative standard deviation was 3.16-6.54%, suggesting the good accuracy of the developed method. Moreover, it exhibited good selectivity, reproducibility, and stability, thus demonstrating the potential significant applications of the electrochemical immunosensor in drinks safety monitoring.
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