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PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis

文献类型： 外文期刊

作者： Cao, Xiao-Tong 1 ; Wu, Lian-Jie 1 ; Xu, Feng-Lin 1 ; Li, Xin-Cang 2 ; Lan, Jiang-Feng 1 ;

作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Vet Med, Shandong Prov Key Lab Anim Biotechnol & Dis Contro, Tai An 271018, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploitat, Shanghai 200090, Peoples R China

关键词： Tripartite motif (TRIM); AP1; White spot syndrome virus; Endocytosis; Dynamin; Immediate-early protein (IE1)

期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.4； 五年影响因子：8.0 ）

ISSN：

年卷期： 2023 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Viruses have evolved various strategies to achieve early infection by initiating transcription of their own early genes via host transcription factors, such as NF-kappa b, STAT, and AP1. How the host copes with this immune escape has been a topic of interest. Tripartite motif (TRIM) family proteins with RING-type domains have E3 ubiquitin ligase activity and are known as host restriction factors. Trim has been reported to be associated with phagocytosis and is also believed to be involved in the activation of autophagy. Preventing the virus from entering the host cell may be the most economical way for the host to resist virus infection. The role of TRIM in the early stage of virus infection in host cells remains to be further interpreted. In the current study, a crayfish TRIM with a RING-type domain, designated as PcTrim, was significantly upregulated under white spot syndrome virus (WSSV) infection in the red swamp crayfish (Procambarus clarkii). Recombinant PcTrim significantly inhibited WSSV replication in crayfish. RNAi targeting PcTrim or blocking PcTrim with an antibody promoted WSSV replication in crayfish. Pulldown and co-IP assays showed that PcTrim can interact with the virus protein VP26. PcTrim restricts the expression level of dynamin, which is involved in the regulation of phagocytosis, by inhibiting AP1 entry into the nucleus. AP1-RNAi effectively reduced the expression levels of dynamin and inhibited host cell endocytosis of WSSV in vivo. Our study demonstrated that PcTrim might reduce early WSSV infection by binding to VP26 and then inhibiting AP1 activation, resulting in reduced endocytosis of WSSV in crayfish hemocytes.

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