文献类型： 外文期刊
作者： Lu, Gang 1 ; Yao, Min 1 ; Zhou, Yijun 4 ; Tao, Xiaorong 1 ;
作者机构： 1.Nanjing Agr Univ, Dept Plant Pathol, Nanjing, Peoples R China
2.Ningbo Univ, Inst Plant Virol, Ningbo, Peoples R China
3.Chinese Acad Sci, Shanghai Inst MateriaMed, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
关键词： Rice stripe virus; Filamentous virion; Density gradient centrifugation; Virion purification; Ultracentrifugation
期刊名称：BIO-PROTOCOL （ 影响因子：0.8； 五年影响因子：1.3 ）
ISSN：
年卷期： 2020 年 10 卷 6 期
页码：
收录情况： SCI
摘要： Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated with polyethylene glycol (PEG) followed by pelleting through primary ultracentrifugation, ultracentrifugation in a glycerol cushion and ultracentrifugation in density gradient. The purified RSV virions can not only be viewed as filamentous particles under an electron microscope, but can also be acquired by insect vector through direct injection into insect body or through membrane feeding prior to transmission to rice plants.
- 相关文献
作者其他论文 更多>>
-
The miR-184-3p promotes rice black-streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)
作者：Wu, Wei;Wang, Man;Deng, Zhiting;Xi, Minmin;Dong, Yan;Wang, Haitao;Zhang, Jianhua;Zhou, Yijun;Xu, Qiufang;Wu, Wei;Deng, Zhiting;Xi, Minmin;Wang, Changchun;Xu, Qiufang
关键词：miR-184-3p; Laodelphax striatellus; rice black-streaked dwarf virus; Ken; virus infection
-
The miR184-3p targets neuron-specific ecdysone inducible protein 78 to promote rice black streaked dwarf virus propagation in its planthopper vector
作者：Wang, Haitao;Dong, Yan;Wang, Man;Li, Shuo;Zhou, Yijun;Ji, Yinghua
关键词：miR184-3p; rice black streak dwarf virus; antiviral mechanism; central nervous system; ecdysone inducible protein 78; ecdysone receptor
-
A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity
作者：Huang, Shen;Wang, Chunli;Ding, Zixuan;Zhao, Yaqian;Dai, Jing;Li, Jia;Huang, Haining;Wang, Tongkai;Zhu, Min;Feng, Mingfeng;Tao, Xiaorong;Ji, Yinghua;Zhang, Zhongkai
关键词：
-
Molecular Hydrogen Confers Resistance to Rice Stripe Virus
作者：Shao, Yudong;Lin, Feng;Wang, Zhaoyun;Liu, Zhiyang;Chen, Dongyue;Guo, Wei;Lan, Ying;Du, Linlin;Zhou, Yijun;Zhou, Tong;Shao, Yudong;Wang, Yueqiao;Cheng, Pengfei;Lou, Wang;Chen, Dongyue;Shen, Wenbiao
关键词：Arabidopsis; disease resistance; molecular hydrogen; rice; rice stripe virus; salicylic acid
-
Development of novel specific molecular markers for the Sw-5b gene to assist with tomato spotted wilt virus-resistant tomato breeding
作者：Tong, Cong;Huang, Shen;Shi, Yajie;Wu, Qian;Shangguan, Lingna;Yu, Haohua;Chen, Rongzhen;Ding, Zixuan;Xiao, Yunxia;Zhu, Min;Tao, Xiaorong;Ji, Yinghua;Zhang, Zhongkai;Zhang, Hui
关键词：Sw-5b; TSWV; Gene-specific molecular marker; Marker-assisted selection; Breeding; Tomato
-
Genetic variability of rice stripe virus after its pandemic in Jiangsu
作者：Lu, Chengye;Miu, Qian;Jin, Daoran;Cheng, Zhaobang;Zhou, Yijun;Li, Shuo;Lu, Chengye;Wang, Yunyue;Li, Aiguo
关键词：Rice stripe virus; Genetic variability; Non-epidemic period; Genotype; NSvc4 variation
-
An improved method to efficiently acquire rice black-streaked dwarf virus viruliferous small brown planthoppers
作者：Du, Linlin;Zhou, Tong;Du, Linlin;Li, Xuejuan;Lan, Ying;Guo, Wei;Wang, Zhaoyun;Liu, Zhiyang;Zhou, Yijun;Zhou, Tong;Du, Linlin;Li, Xuejuan;Lan, Ying;Guo, Wei;Wang, Zhaoyun;Liu, Zhiyang;Zhou, Yijun;Zhou, Tong;Zeng, Bo;Jiban, Kumar Kunda
关键词：RBSDV; viruliferous SBPHs; feeding times; RT-qPCR; virus loads
