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Resistance gene Ty-1 restricts TYLCV infection in tomato by increasing RNA silencing

文献类型： 外文期刊

作者： Ma, Xiaofang 1 ; Zhou, Yijun 2 ; Wu, Liming 1 ; Moffett, Peter 3 ;

作者机构： 1.Hubei Acad Agr Sci, Res Inst Fruit & Tea, Hubei Key Lab Germplasm Innovat & Utilizat Fruit T, Wuhan 430064, Hubei, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

3.Univ Sherbrooke, Ctr SEVE, Dept Biol, 2500 Blvd Univ, Sherbrooke, PQ J1K 2R1, Canada

关键词： RNA silencing; Nicotiana benthamiana; Ty-1; RDRs proteins; TYLCV V2

期刊名称：VIROLOGY JOURNAL （ 影响因子：3.8； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 21 卷 1 期

页码：

收录情况： SCI

摘要： A major antiviral mechanism in plants is mediated by RNA silencing through the action of DICER-like (DCL) proteins, which cleave dsRNA into discrete small RNA fragments, and ARGONAUTE (AGO) proteins, which use the small RNAs to target single-stranded RNA. RNA silencing can also be amplified through the action of RNA-dependent RNA polymerases (RDRs), which use single stranded RNA to generate dsRNA that in turn is targeted by DCL proteins. As a counter-defense, plant viruses encode viral suppressors of RNA silencing (VSRs) that target different components in the RNA silencing pathway. The tomato Ty-1 gene confers resistance to the DNA virus tomato yellow leaf curl virus (TYLCV) and has been reported to encode an RDR gamma protein. However, the molecular mechanisms by which Ty-1 controls TYLCV infection, including whether Ty-1 is involved in RNA silencing, are unknown. Here, by using a transient expression assay, we have confirmed that Ty-1 shows antiviral activity against TYLCV in Nicotiana benthamiana. Also, in transient expression-based silencing assays, Ty-1 augmented systemic transgene silencing in GFP transgenic N. benthamiana plants. Furthermore, co-expression of Ty-1 or other RDR gamma proteins from N. benthamiana or Arabidopsis with various proteins resulted in lower protein expression. These results are consistent with a model wherein Ty-1-mediated resistance to TYLCV is due, at least in part, to an increase in RNA silencing activity.

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