您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

4D genetic networks reveal the genetic basis of metabolites and seed oil-related traits in 398 soybean RILs

文献类型： 外文期刊

作者： Han, Xu 1 ; Zhang, Ya-Wen 1 ; Liu, Jin-Yang 2 ; Zuo, Jian-Fang 1 ; Zhang, Ze-Chang 1 ; Guo, Liang 1 ; Zhang, Yuan-Ming 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

关键词： Multi-dimension genetic network; Lipid; Metabolite; miRNA; Seed oil-related trait; Recombinant inbred line; Soybean

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

ISSN：

年卷期： 2022 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Background The yield and quality of soybean oil are determined by seed oil-related traits, and metabolites/lipids act as bridges between genes and traits. Although there are many studies on the mode of inheritance of metabolites or traits, studies on multi-dimensional genetic network (MDGN) are limited. Results In this study, six seed oil-related traits, 59 metabolites, and 107 lipids in 398 recombinant inbred lines, along with their candidate genes and miRNAs, were used to construct an MDGN in soybean. Around 175 quantitative trait loci (QTLs), 36 QTL-by-environment interactions, and 302 metabolic QTL clusters, 70 and 181 candidate genes, including 46 and 70 known homologs, were previously reported to be associated with the traits and metabolites, respectively. Gene regulatory networks were constructed using co-expression, protein-protein interaction, and transcription factor binding site and miRNA target predictions between candidate genes and 26 key miRNAs. Using modern statistical methods, 463 metabolite-lipid, 62 trait-metabolite, and 89 trait-lipid associations were found to be significant. Integrating these associations into the above networks, an MDGN was constructed, and 128 sub-networks were extracted. Among these sub-networks, the gene-trait or gene-metabolite relationships in 38 sub-networks were in agreement with previous studies, e.g., oleic acid (trait)-GmSEI-GmDGAT1a-triacylglycerol (16:0/18:2/18:3), gene and metabolite in each of 64 sub-networks were predicted to be in the same pathway, e.g., oleic acid (trait)-GmPHS-d-glucose, and others were new, e.g., triacylglycerol (16:0/18:1/18:2)-GmbZIP123-GmHD-ZIPIII-10-miR166s-oil content. Conclusions This study showed the advantages of MGDN in dissecting the genetic relationships between complex traits and metabolites. Using sub-networks in MGDN, 3D genetic sub-networks including pyruvate/threonine/citric acid revealed genetic relationships between carbohydrates, oil, and protein content, and 4D genetic sub-networks including PLDs revealed the relationships between oil-related traits and phospholipid metabolism likely influenced by the environment. This study will be helpful in soybean quality improvement and molecular biological research.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台