文献类型： 外文期刊

作者： Zhao Xiangxang ¹ ; Luo Yuming ¹ ; Lu Weiping ² ; Qi Cunkou ³ ; Pu Huiming ³ ; Wang Youping ² ;

作者机构： 1.Huaiyin Normal Univ, Biotechnol Key Lab Eco Agr Hongze Lake Reg, Huainan 223001, Peoples R China

2.Yangzhou univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Yangzhou 225009, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

关键词： herbicide-resistant rapeseed;cruciferous weeds;mentor pollination;aniline blue fluorescence;gene flow.

期刊名称：PROGRESS IN NATURAL SCIENCE （ 影响因子：0.704； 五年影响因子：0.688 ）

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收录情况： SCI

摘要： The alien gene flow between genetically modified glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and four cruciferous weeds was studied under mentor pollen inducement. The results showed that when Thlaspi arvense L., Capsella bursa-pastoris (L. ) Medic, Cardamine hirsuta L. and Rorippa palustris (L. ) Besser were pollinated with mentor pollen, the mixed Q3 and the weed, pollen grains aggregated largely and germinated quickly, and the numbers of pollen tubes penetrating into the style andthe ovary were greatly increased as compared with corresponding self-pollination groups. Twenty four to forty eight hours after pollination, several pollen tubes were observed to penetrate into the ovule via micropyle in each mentor combination. However, when the mentor progenies were analyzed by PCR, all of them showed negative for the Q3 herbicide-resistant gene. Collectively, these results indicated that crossing between T. arvense, C. bursa-pastoris, C. hirsuta, R. palustris (as female) and Q3 (asmale) was highly incompatible and the herbicide-resistant gene could not flow from Q3 to these four weeds.