您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen

文献类型： 外文期刊

作者： Wang, Wei 1 ; Li, Jizong 1 ; Fan, Baochao 1 ; Zhang, Xuehan 1 ; Guo, Rongli 1 ; Zhao, Yongxiang 1 ; Zhou, Junming 1 ; Zhou, Jinzhu 1 ; Sun, Dongbo 5 ; Li, Bin 1 ;

作者机构： 1.Inst Vet Med, Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing 210014, Peoples R China

2.Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Peoples R China

3.Jiangsu CoInfect Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

4.Yangzhou Univ, Jiangsu Key Lab Zoonoses, Yangzhou 225000, Jiangsu, Peoples R China

5.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Lab Prevent & Control Swine Infect Dis, Daqing 163319, Peoples R China

关键词： porcine deltacoronavirus; quantitative ELISA; antigen detection; intestinal and fecal samples; vaccine evaluation

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2021 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the PDCoV N protein and an anti-PDCoV rabbit polyclonal antibody. Using DAS-ELISA, the detection limit of recombinant PDCoV N protein and virus titer were approximately 0.5 ng/mL and 10(3.0) TCID50/mL, respectively. A total of 59 intestinal and 205 fecal samples were screened for the presence of PDCoV by using DAS-ELISA and reverse transcriptase real-time PCR (RT-qPCR). The coincidence rate of the DAS-ELISA and RT-qPCR was 89.8%. DAS-ELISA had a sensitivity of 80.8% and specificity of 95.6%. More importantly, the DAS-ELISA could detect the antigen of PDCoV inactivated virus, and the viral antigen concentrations remained unchanged in the inactivated virus. These results suggest that DAS-ELISA could be used for antigen detection of clinical samples and inactivated vaccines. It is a novel method for detecting PDCoV infections and evaluating the PDCoV vaccine.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台