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A novel approach to analyze the association characteristics between post-spliced introns and their corresponding mRNA

文献类型： 外文期刊

作者： Bo, Suling 1 ; Sun, Qiuying 2 ; Ning, Pengfei 1 ; Yuan, Ningping 1 ; Weng, Yujie 1 ; Liang, Ying 1 ; Wang, Huitao 1 ; Lu, Zhanyuan 4 ; Li, Zhongxian 1 ; Zhao, Xiaoqing 4 ;

作者机构： 1.Inner Mongolia Med Univ, Coll Comp Informat, Hohhot, Peoples R China

2.Inner Mongolia Med Univ, Affiliated Peoples Hosp, Hohhot, Peoples R China

3.Inner Mongolia Med Univ, Inner Mongolia Canc Hosp, Dept Oncol, Hohhot, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

5.Inner Mongolia Univ, Sch Life Sci, Hohhot, Peoples R China

6.Minist Agr & Rural Affairs, Key Lab Black Soil Protection & Utilizat Hohhot, Hohhot, Peoples R China

7.Inner Mongolia Key Lab Degradat Farmland Ecol Rest, Hohhot, Peoples R China

关键词： intron; alignment method; interaction; mRNA; evolutionary process

期刊名称：FRONTIERS IN GENETICS （ 影响因子：3.7； 五年影响因子：4.3 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Studies have shown that post-spliced introns promote cell survival when nutrients are scarce, and intron loss/gain can influence many stages of mRNA metabolism. However, few approaches are currently available to study the correlation between intron sequences and their corresponding mature mRNA sequences. Here, based on the results of the improved Smith-Waterman local alignment-based algorithm method (SW method) and binding free energy weighted local alignment algorithm method (BFE method), the optimal matched segments between introns and their corresponding mature mRNAs in Caenorhabditis elegans (C.elegans) and their relative matching frequency (RF) distributions were obtained. The results showed that although the distributions of relative matching frequencies on mRNAs obtained by the BFE method were similar to the SW method, the interaction intensity in 5'and 3'untranslated regions (UTRs) regions was weaker than the SW method. The RF distributions in the exon-exon junction regions were comparable, the effects of long and short introns on mRNA and on the five functional sites with BFE method were similar to the SW method. However, the interaction intensity in 5'and 3'UTR regions with BFE method was weaker than with SW method. Although the matching rate and length distribution shape of the optimal matched fragment were consistent with the SW method, an increase in length was observed. The matching rates and the length of the optimal matched fragments were mainly in the range of 60%-80% and 20-30bp, respectively. Although we found that there were still matching preferences in the 5'and 3'UTR regions of the mRNAs with BFE, the matching intensities were significantly lower than the matching intensities between introns and their corresponding mRNAs with SW method. Overall, our findings suggest that the interaction between introns and mRNAs results from synergism among different types of sequences during the evolutionary process.

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