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Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Lu, Yi-Fang 1 ; Liu, Qian 2 ; Liu, Kai-Qiang 2 ; Wang, Hong-Yan 2 ; Li, Cheng-Hua 1 ; Wang, Qian 2 ; Shao, Chang-Wei 2 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Shandong, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Shandong, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Gonadal differentiation; Alternative splicing; Sex-specific splicing; Cynoglossus semilaevis; Iso-Seq; RNA-Seq

期刊名称：ZOOLOGICAL RESEARCH （ 影响因子：6.975； 五年影响因子：4.811 ）

ISSN： 2095-8137

年卷期： 2022 年 43 卷 3 期

页码：

收录情况： SCI

摘要： Chinese tongue sole (Cynoglossus semilaevis) is an economically important marine fish species with a ZZ/ZW sex determination mechanism, which can be influenced by temperature. Alternative splicing (AS) is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation, but has rarely been reported in fish. In this study, to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation, we combined isoform and RNA sequencing (Iso-Seq and RNA-Seq) to perform transcriptome profiling of male and female gonads in C. semilaevis. In total, 81 883 and 32 341 full-length transcripts were obtained in males and females, respectively. A total of 8 279 AS genes were identified, including 2 639 genes showing differential AS (DAS) between males and females. Many intersecting DAS genes and differentially expressed genes (DEGs) were enriched in the meiotic cell cycle pathway, and genes related to gonadal differentiation, such as esrrb and wt1a, were found to have sex-specific isoforms. Thus, this study revealed AS events in the gonadal transcriptomes of male and female C. semilaevis, described the characteristics of active transcription in the testes, and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation.

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