文献类型： 外文期刊

作者： Song, Weiyuan ¹ ; Xie, Yang ² ; Liu, Bin ³ ; Huang, Yuxiang ¹ ; Cheng, Zhihua ¹ ; Zhao, Zilong ⁴ ; Tian, Di ¹ ; Geng, Yan ¹ ; Guo, Jingyu ¹ ; Li, Chuang ¹ ; She, Daixi ¹ ; Zhong, Yanting ¹ ; Li, Min ¹ ; Liu, Liu ¹ ; Chen, Jiacai ¹ ; Sun, Chengzhen ² ; Zhang, Xuejun ³ ; Zhou, Zhaoyang ¹ ; Lai, Jinsheng ⁴ ; Xin, Ming ⁵ ; Yan, Liying ² ; Zhao, Jianyu ¹ ; Zhang, Xiaolan ¹ ;

作者机构： 1.China Agr Univ, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Veg, Beijing 100193, Peoples R China

2.Hebei Normal Univ Sci & Technol, Hebei Key Lab Hort Germplasm Excavat & Innovat Uti, Qinhuangdao 066004, Peoples R China

3.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Urumqi 830091, Peoples R China

4.China Agr Univ, Dept Plant Genet & Breeding, State Key Lab Maize Biobreeding, Natl Maize Improvement Ctr,Frontiers Sci Ctr Mol D, Beijing 100193, Peoples R China

5.Northeast Agr Univ, Coll Hort & Landscape Architecture, Key Lab Biol & Genet Improvement Hort Crops Northe, Harbin 150030, Peoples R China

期刊名称：PLANT CELL （ 影响因子：10.0； 五年影响因子：11.1 ）

ISSN： 1040-4651

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite to fulfill breeding potential. Here, we found 2 SNPs in the E-class floral organ identity gene cucumber (Cucumis sativus) SEPALLATA2 (CsSEP2) that specifically regulate fruit length. Haplotype (HAP) 1 (8G2667A) and HAP2 (8G2667T) exist in natural populations, whereas HAP3 (8A2667T) is induced by ethyl methanesulfonate mutagenesis. Phenotypic characterization of 4 near-isogenic lines and a mutant line showed that HAP2 fruits are significantly longer than those of HAP1, and those of HAP3 are 37.8% longer than HAP2 fruit. The increasing fruit length in HAP1-3 was caused by a decreasing inhibitory effect on CRABS CLAW (CsCRC) transcription (a reported positive regulator of fruit length), resulting in enhanced cell expansion. Moreover, a 7638G/A-SNP in melon (Cucumis melo) CmSEP2 modulates fruit length in a natural melon population via the conserved SEP2-CRC module. Our findings provide a strategy for utilizing essential regulators with pleiotropic effects during crop breeding. Single-nucleotide changes in a flower development-related gene contribute to fruit length variations in cucurbits via differential inhibitory effects on transcription and cell expansion.