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A nanopore-based cucumber genome assembly reveals structural variations at two QTLs controlling hypocotyl elongation

文献类型： 外文期刊

作者： Liu, Bin 1 ; Shen, Cheng-Cheng 4 ; Xia, Shi-Wei 1 ; Song, Shan-Shan 1 ; Su, Li-Hong 1 ; Li, Yu 1 ; Hao, Qian 1 ; Liu, Yan-Jun 1 ; Guan, Dai-Lu 1 ; Wang, Ning 1 ; Wang, Wen-Jiao 1 ; Zhao, Xiang 1 ; Li, Huan-Xiu 1 ; Li, Xi-Xiang 5 ; Lai, Yun-Song 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Hort, Chengdu 611130, Peoples R China

2.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Urumqi 830091, Peoples R China

3.Campus Univ Autonoma Barcelona, CSIC IRTA UAB UB, Ctr Res Agr Genom CRAG, Bellaterra 08193, Spain

4.Shanxi Agr Univ, Coll Hort, Jinzhong 030801, Peoples R China

5.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100080, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2024 年 195 卷 2 期

页码：

收录情况： SCI

摘要： The Xishuangbanna (XIS) cucumber (Cucumis sativus var. xishuangbannanesis) is a semiwild variety that has many distinct agronomic traits. Here, long reads generated by Nanopore sequencing technology helped assembling a high-quality genome (contig N50 = 8.7 Mb) of landrace XIS49. A total of 10,036 structural/sequence variations (SVs) were identified when comparing with Chinese Long (CL), and known SVs controlling spines, tubercles, and carpel number were confirmed in XIS49 genome. Two QTLs of hypocotyl elongation under low light, SH3.1 and SH6.1, were fine-mapped using introgression lines (donor parent, XIS49; recurrent parent, CL). SH3.1 encodes a red-light receptor Phytochrome B (PhyB, CsaV3_3G015190). A similar to 4 kb region with large deletion and highly divergent regions (HDRs) were identified in the promoter of the PhyB gene in XIS49. Loss of function of this PhyB caused a super-long hypocotyl phenotype. SH6.1 encodes a CCCH-type zinc finger protein FRIGIDA-ESSENTIAL LIKE (FEL, CsaV3_6G050300). FEL negatively regulated hypocotyl elongation but it was transcriptionally suppressed by long terminal repeats retrotransposon insertion in CL cucumber. Mechanistically, FEL physically binds to the promoter of CONSTITUTIVE PHOTOMORPHOGENIC 1a (COP1a), regulating the expression of COP1a and the downstream hypocotyl elongation. These above results demonstrate the genetic mechanism of cucumber hypocotyl elongation under low light. Structural variation in quantitative trait loci affects hypocotyl elongation in Xishuangbanna cucumber.

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