Down-Regulation of Lipid Metabolism in the Hepatopancreas of Shrimp Litopenaeus vannamei upon Light and Heavy Infection of Enterocytozoon hepatopenaei: A Comparative Proteomic Study
文献类型： 外文期刊
作者： Wu, Yujiao 1 ; Chen, Jie 1 ; Liao, Guoli 3 ; Hu, Mengjiao 1 ; Zhang, Qing 1 ; Meng, Xianzhi 1 ; Li, Tian 1 ; Long, Mengxian 1 ; Fan, Xiaodong 4 ; Yu, Qing 1 ; Zhang, Liping 5 ; Pan, Guoqing 1 ; Zhou, Zeyang 1 ;
作者机构： 1.Southwest Univ, Chongqing Key Lab Microsporidia Infect & Control, Chongqing 400715, Peoples R China
2.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China
4.Chongqing Normal Univ, Coll Life Sci, Chongqing 400047, Peoples R China
5.Chongqing Fisheries Tech Extens Ctr, Chongqing 400014, Peoples R China
关键词： Enterocytozoon hepatopenaei; Litopenaeus vannamei; proteomic; lipid metabolism
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 19 期
页码：
收录情况： SCI
摘要： Enterocytozoon hepatopenaei (EHP) is the pathogen of hepatopancreatic microsporidiosis (HPM) in shrimp. The diseased shrimp Litopenaeus vannamei exhibits a slow growth syndrome, which causes severe economic losses. Herein, 4D label-free quantitative proteomics was employed to analyze the hepatopancreas of L. vannamei with a light (EHPptp2 < 10(3) copies/50 ng hpDNA, L group) and heavy (EHPptp2 > 10(4) copies/50 ng hpDNA, H group) load of EHP to better understand the pathogenesis of HPM. Exactly 786 (L group) and 1056 (H group) differentially expressed proteins (DEPs) versus the EHP-free (C group) control were mainly clustered to lipid metabolism, amino acid metabolism, and energy production processing. Compared with the L group, the H group exhibited down-regulation significantly in lipid metabolism, especially in the elongation and degradation of fatty acid, biosynthesis of unsaturated fatty acid, metabolism of alpha-linolenic acid, sphingolipid, and glycerolipid, as well as juvenile hormone (JH) degradation. Expression pattern analysis showed that the degree of infection was positively correlated with metabolic change. About 479 EHP proteins were detected in infected shrimps, including 95 predicted transporters. These findings suggest that EHP infection induced the consumption of storage lipids and the entire down-regulation of lipid metabolism and the coupling energy production, in addition to the hormone metabolism disorder. These were ultimately responsible for the stunted growth.
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