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The PLA Gene Family in Tomato: Identification, Phylogeny, and Functional Characterization

文献类型： 外文期刊

作者： Li, Zixing 1 ; Yao, Zhuping 2 ; Ruan, Meiying 2 ; Wang, Rongqing 2 ; Ye, Qingjing 2 ; Wan, Hongjian 2 ; Zhou, Guozhi 2 ; Cheng, Yuan 2 ; Guo, Shangjing 4 ; Liu, Chaochao 1 ; Liu, Chenxu 2 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212018, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Vegetables, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Zhejiang Xianghu Lab, Hangzhou 311258, Peoples R China

4.Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China

关键词： phospholipase A; genome-wide identification; phylogenetic analysis; cold stress; tomato

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Background: Phospholipase A (PLA) enzymes catalyze the hydrolysis of glycerophospholipids, releasing free fatty acids and lysophospholipids that play vital roles in plant growth, development, and stress responses. Methods: This study identified and analyzed SlPLA genes through bioinformatics and further explored the function of PLA genes under cold stress through virus-induced gene silencing (VIGS) experiments. Results: This study systematically characterized the SlPLA gene family in tomato, identifying 80 genes distributed across 12 chromosomes. Phylogenetic analysis categorized these genes into three groups: pPLA, PLA1, and PLA2. Conserved motifs and gene structure analysis revealed distinct patterns, with some genes lacking untranslated regions (UTRs), which suggests functional diversification. Promoter analysis indicated that SlPLA genes are regulated by light, hormones, and stress-related elements, particularly cold stress. RNA-seq data and qRT-PCR results indicated the differential expression of SlPLA genes across various tissues in tomato cultivars (Heinz and Micro-Tom). Under cold stress, certain SlPLA genes, especially SlPLA1-2, were up-regulated, suggesting their involvement in cold tolerance. Silencing SlPLA1-2 resulted in increased membrane damage, elevated malondialdehyde (MDA) levels, higher electrolyte leakage, and a lower expression of cold-responsive genes within the ICE1-CBF-COR pathway and jasmonic acid (JA) biosynthesis. Conclusions: This study discovered 80 SlPLA genes in tomato across 12 chromosomes, categorizing them into pPLA, PLA1, and PLA2 via phylogenetic analysis. The qRT-PCR analysis identified that SlPLA1-2 was strongly induced by cold stress, and further experiments regarding genetics and physiology revealed that SlPLA1-2 boosts the cold tolerance of tomato by affecting the CBF signaling pathway and JA biosynthesis, offering insights for future stress-resilience breeding.

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