文献类型： 外文期刊

作者： Wang, Zhen ¹ ; Xia, Mingzhe ² ; Ma, Rui ³ ; Zheng, Zai ⁴ ;

作者机构： 1.Open Univ China, Sch Agr Forestry & Med, Beijing, Peoples R China

2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China

3.Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, Minist Educ,Key Lab Bioinformat, Beijing, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding, Hainan, Peoples R China

5.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan, Peoples R China

关键词： Pi starvation; light illumination; primary root growth; transcriptomic analyses; cryptochromes; HY5

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Plants cope with Pi deficiency by triggering an array of adaptive responses, including the remodeling of root system architecture (RSA). Arabidopsis thaliana grown on a Pi-deficient (-Pi) medium in transparent Petri dishes exhibits an inhibition of primary root (PR) growth. Previous work has shown that direct illumination on roots by blue light is both required and sufficient for the Pi deficiency-induced inhibition of PR growth. However, whether light illumination on shoots of seedlings contributes to the inhibition of PR growth under -Pi condition and whether light signaling pathway is involved in this process remain largely unknown. In addition to Pi deficiency-induced inhibition of PR growth, how light affects the transcriptomic changes under -Pi also remains elusive. Here, we found that the inhibition of PR growth under -Pi condition is determined by light illumination on roots instead of shoots. Further experiments revealed that blue light receptors CRY1/CRY2 and key regulator in blue light signaling pathway HY5 play minor roles in this process. Finally, we evaluated the light effects on the transcriptomic changes during the inhibition of PR growth under -Pi condition. We found that light promotes the expression of many genes involved in stress and phytohormones-related processes and has both upregulated and downregulated effects on the expression of typical phosphate starvation-induced (PSI) genes. Taken together, our work further demonstrates our previous hypothesis that the inhibition of PR growth under -Pi condition is caused by blue light-triggered chemical reactions, rather than blue light signaling pathways. Apart from the inhibition of PR growth under -Pi, light exposure also results in substantial alterations of transcriptome under -Pi condition, encouraging us to carefully evaluate the phenotype under illuminated, transparent Petri dishes.