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POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes

文献类型： 外文期刊

作者： Zhang, Geyang 1 ; Wang, Jiamei 3 ; Li, Yulong 1 ; Zhang, Zijing 1 ; Wang, Xiangnan 1 ; Chen, Fuying 1 ; Shi, Qiaoting 1 ; Huang, Yongzhen 3 ; Wang, Eryao 1 ; Lyu, Shijie 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450046, Peoples R China

3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

4.Shennong Lab, Zhengzhou 450002, Peoples R China

关键词： POLB; myoblasts; RNA-seq; qPCR; analysis

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 9 期

页码：

收录情况： SCI

摘要： This study focused on the DNA polymerase beta (POLB) gene, which belongs to the DNA polymerase X family involved in DNA replication, repair, recombination, and cell cycle regulation. POLB was identified as a muscle development-related gene through gene screening and gene expression analysis methods. Further validation in bovine primary myocytes showed that overexpression of POLB promoted apoptosis, while gene knockdown had no significant effect. Analysis of related genes revealed that POLB overexpression affected the expression of the CASP9 gene, which is involved in the apoptotic pathway. DNA polymerase beta (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression.

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