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In Vitro Analysis of LPS-Induced miRNA Differences in Bovine Endometrial Cells and Study of Related Pathways

文献类型： 外文期刊

作者： Li, Xinmiao 1 ; Zhang, Zhihao 1 ; Wang, Xiangnan 1 ; Lu, Ligang 4 ; Zhang, Zijing 1 ; Zhang, Geyang 3 ; Min, Jia 3 ; Shi, Qiaoting 1 ; Lyu, Shijie 1 ; Chu, Qiuxia 1 ; Qi, Xingshan 5 ; Li, Huimin 6 ; Huang, Yongzhen 2 ; Wang, Eryao 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb, Zhengzhou 450002, Peoples R China

2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China

4.Bijie Acad Agr Sci, Bijie 551700, Peoples R China

5.Bur Anim Husb Biyang Cty, Zhumadian 463700, Biyang, Peoples R China

6.Agr Comprehens Adm Law Enforcement Detachment Zhen, Zhengzhou 450044, Peoples R China

关键词： LPS; endometritis; inflammatory models; miRNA

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 23 期

页码：

收录情况： SCI

摘要： Lipopolysaccharide (LPS) is one of the main factors inducing endometritis in dairy cows. However, the specific pathogenesis of LPS-induced endometritis in dairy cows is not fully understood. The objective of this study was to establish an in vitro endometritis model using LPS-induced bovine endometrial epithelial (BEND) cells. BEND cells were treated with LPS of different concentrations and times. The cell-counting kit-8 (CCK-8) was used to detect the cell survival rate after LPS treatment, and quantitative real-time PCR (RT-qPCR) was used to detect the expression of control group and LPS-treated group of inflammatory factors interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-alpha). The results showed that the survival rate of endometrial epithelial cells stimulated by 5 mu g/mL LPS for 6 h was 75.13%, and the expression of inflammatory factors was significantly increased. Therefore, 5 mu g/mL LPS for 6 h could be selected as a suitable model for the study of inflammation. In addition, miRNA sequencing and target gene prediction was performed on normal and LPS-treated BEND cells. Among twenty-one differentially expressed miRNAs, six miRNAs were selected and their expression levels were detected by RT-qPCR, which were consistent with the sequencing results. Twenty-one differentially expressed miRNAs collectively predicted 17,050 target genes. This study provides a theoretical basis for further investigation of the pathogenesis of endometritis.

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