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Screening and Analysis of Antifungal Strains Bacillus subtilis JF-4 and B. amylum JF-5 for the Biological Control of Fusarium Wilt of Banana

文献类型： 外文期刊

作者： Duan, Yajie 1 ; Pang, Zhencai 1 ; Yin, Shunli 4 ; Xiao, Weijun 1 ; Hu, Huigang 1 ; Xie, Jianghui 1 ; Moussa, El Jarroudi 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr & Rural Affairs, Zhanjiang 524091, Peoples R China

2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Hainan Prov Postharvest Physiol & Technol, Zhanjiang 524091, Peoples R China

3.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China

4.Kunming Univ, Coll Agr & Life Sci, Kunming 650214, Peoples R China

关键词： Fusarium wilt of banana; antagonistic strain; inhibition mechanism; biocontrol application; secondary metabolite; Bacillus

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 9 期

页码：

收录情况： SCI

摘要： Purpose: This study aimed to identify the antagonistic bacteria from the rhizosphere of healthy bananas that can effectively suppress the Fusarium wilt of banana, and to further investigate the inhibitory mechanism. Method: The primary and secondary screening techniques were implemented using the double-plate and fermentation antagonism methods. The strain was identified based on physiological and biochemical tests, 16S rRNA gene sequencing, and specific gene amplification. The effects of crude extract on the protein content, lipid peroxidation, and pectinase activity of mycelia were determined from the identified isolates. Results: Two antagonistic bacteria, JF-4 and JF-5, were screened and initially identified as Bacillus subtilis (GenBank: OR125631) and B. amylum (GenBank: OR125632). The greenhouse experiment showed that the biological control efficiency of the two antagonists against the Fusarium wilt of banana was 48.3% and 40.3%, respectively. The catalase content produced by lipid peroxidation increased significantly after treatment with the crude extracts of JF-4 and JF-5 at concentrations of 0.69 mu mol/L and 0.59 mu mol/L, respectively. The protein and ergosterol content and pectinase activity decreased significantly. The two antagonistic bacteria might inhibit the growth of pathogens by enhancing lipid peroxidation and decreasing the synthesis of cell metabolites. Twenty compounds were identified by gas chromatography-mass spectrometry (GC-MS). B. subtilis JF-4 was further sequenced and assembled to obtain a complete circular chromosome genome of 681,804,824 bp. The genome consisted of a 4,310,825-bp-long scaffold. Conclusion: The findings of this study may help elucidate the mechanism behind this biocontrol isolate.

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