Potential antagonistic relationship of fgf9 and rspo1 genes in WNT4 pathway to regulate the sex differentiation in Chinese giant salamander (Andrias davidianus)
文献类型： 外文期刊
作者： Zhang, Jiankang 1 ; Xia, Xueping 1 ; Zhu, Ying 2 ; Lian, Zitong 1 ; Tian, Haifeng 1 ; Xiao, Hanbing 1 ; Hu, Qiaomu 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China
关键词： Andrias davidianus; FGF9; rspo1; antagonize; sex differentiation
期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：6.113； 五年影响因子：6.122 ）
ISSN：
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： Farmed chinese giant salamander (Andrias davidianus) was an important distinctive economically amphibian that exhibited male-biased sexual size dimorphism. Fgf9 and rspo1 genes antagonize each other in Wnt4 signal pathway to regulate mammalian gonadal differentiation has been demonstrated. However, their expression profile and function in A. davidianus are unclear. In this study, we firstly characterized fgf9 and rspo1 genes expression in developing gonad. Results showed that fgf9 expression level was higher in testes than in ovaries and increased from 1 to 6 years while rspo1 expression was higher in ovaries than in testes. In situ hybridization assay showed that both fgf9 and rspo1 genes expressed at 62 dpf in undifferentiated gonad, and fgf9 gene was mainly expressed in spermatogonia and sertoli cells in testis while strong positive signal of rspo1 was detected in granular cell in ovary. During sex-reversal, fgf9 expression was significantly higher in reversed testes and normal testes than in ovaries, and opposite expression pattern was detected for rspo1. When FH535 was used to inhibit Wnt/beta-catenin pathway, expression of rspo1, wnt4 and beta-catenin was down-regulated. Conversely, expression of fgf9, dmrt1, ftz-f1 and cyp17 were up-regulated. Furthermore, when rspo1 and fgf9 were knocked down using RNAi technology, respectively. We observed that female biased genes were down regulated in ovary primordial cells after rspo1 was knocked down, while the opposite expression profile was observed in testis primordial cells after fgf9 was knocked down. These results suggested that fgf9 and rspo1 played an antagonistic role to regulate sex differentiation in the process of the gonadal development and provided a foundation for further functional characterizations. The data also provided basic information for genome editing breeding to improve the Chinese giant salamander farming industry.
- 相关文献
作者其他论文 更多>>
-
The potential role of hippo pathway effector yap1/yap1b in female-biased sexual size dimorphism of Chinese tongue sole (Cynoglossus semilaevis)
作者：Mai, Jiaqi;Mai, Jiaqi;Sun, Yuqi;Li, Xihong;Xu, Wenteng;Chen, Zhangfan;Zhang, Qi;Wang, Jiacheng;Wang, Na;Li, Xihong;Xu, Wenteng;Chen, Zhangfan;Wang, Na;Zhu, Ying
关键词：Cynoglossus semilaevis; Sexual size dimorphism; Hippo signaling pathway; yap1; yap1b
-
Diversification of Toll-like receptor 1 in swamp eel ( Monopterus albus )
作者：Rao, Han;Huo, Caifei;Li, Yan;Tian, Haifeng;Li, Zhong;Wang, Xueting;Zhu, Lilan
关键词：Monopterus albus; Toll -like receptor 1 (TLR1); Monopterus javanensis; Genetic diversity
-
Growth arrest and DNA damage-inducible 45 ab gene targeted by miR-20b-5p executes the inflammation and apoptosis by regulating MAPK and NF-κB pathways in black rockfish
作者：Gao, Chengbin;Cai, Xin;Zhu, Ying;Cao, Min;Li, Chao;Gao, Chengbin;Cai, Xin;Ma, Le;Sun, Peng
关键词：Ss _ gadd45ab; Inflammation; Apoptosis; Black rockfish; Regulatory mechanism
-
Genetic diversity of Toll-like receptor 9 in swamp eels (Monopterus albus)
作者：Huo, Caifei;Rao, Han;Zeng, Bo;Li, Debing;Li, Yan;Tian, Haifeng;Li, Zhong;Zeng, Bo;Li, Yan;Zeng, Bo;Li, Yan;Zeng, Bo;Li, Yan
关键词：genetic diversity; Monopterus albus; Monopterus javanensis; Toll-like receptor 9 (TLR9)
-
Genome-wide analysis of BMP/GDF family and DAP-seq of YY1 suggest their roles in Cynoglossus semilaevis sexual size dimorphism
作者：Shi, Rui;Li, Xihong;Xu, Xiwen;Chen, Zhangfan;Wang, Na;Zhu, Ying
关键词：Sexual size dimorphism; Chinese tongue sole ( Cynoglossus semilaevis ); BMP/GDF family
-
Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterusalbus
作者：Hu, Qiaomu;Xia, Xueping;Lian, Zitong;Tian, Haifeng;Li, Zhong
关键词：Monopterusalbus; LncRNA; DNA methylation; Sex reversal; Gene expression
-
Two Nipped-B-Like Protein A (Nipbla) Gametologs in Chinese Tongue Sole (Cynoglossus semilaevis): The Identification of Alternative Splicing, Expression Pattern, and Promoter Activity Analysis
作者：Zhu, Ying;Shi, Rui;Yang, Qian;Zhang, Mengqian;Chen, Songlin;Wang, Na;Shi, Rui;Yang, Qian;Chen, Songlin;Wang, Na;Chen, Songlin;Wang, Na
关键词：nipbla; sex-biased expression; Chinese tongue sole (Cynoglossus semilaevis); sexual size dimorphism; gametolog
