Rapid and quantitative on-site detection of SARS-CoV-2, influenza A, and influenza B viruses using latex microspheres-based immunochromatography with smartphone integration
文献类型： 外文期刊
作者： Li, Peng 1 ; Li, Jiahao 2 ; Jin, Lan 1 ; Zhao, Yuting 1 ; Wu, Xuping 3 ; Liu, Fei 1 ; Shan, Yanke 1 ;
作者机构： 1.Nanjing Agr Univ, Sanya Inst, Joint Int Res Lab Anim Hlth & Food Safety Minist E, Single Mol Biochem & Biomed Lab, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China
3.Nanjing Univ Chinese Med, Hosp Nanjing 2, Nanjing 210003, Jiangsu, Peoples R China
关键词： Multiple respiratory viruses; Immunochromatography test strip; Latex microspheres; Differential diagnosis; Point-of-care testing; Smartphone-assisted detection
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 215 卷
页码：
收录情况： SCI
摘要： Distinguishing between coronavirus disease 2019 (COVID-19) and influenza infections is crucial for effective clinical management and controlling the spread of these diseases, particularly during peak flu seasons when symptoms overlap. Here, we report a rapid on-site detection platform for the simultaneous quantification of antigens from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A (FluA) virus, and influenza B (FluB) virus nucleocapsid protein (NP) antigens in biological samples. The platform integrates latex microspheres-based lateral flow immunoassay (LM-LFIA) with a smartphone-assisted portable reader and a selfprogramming application for rapid quantification. Enabled through quadratic equation fitting, the proposed method achieves quantitative measurement ranges of 0.265-530 ng/mL for SARS-CoV-2 NP, 0.125-50 ng/mL for FluA virus NP, and 0.125-50 ng/mL for FluB virus NP, all with high accuracy. Detection limits were 0.265 ng/mL for SARS-CoV-2 NP, 0.125 ng/mL for FluA virus NP, and 0.125 ng/mL for FluB virus NP. This method demonstrated approximately tenfold higher sensitivity than traditional gold nanoparticles (AuNPs)-based assays and exhibited excellent specificity with no cross-reactivity against other respiratory pathogens. The LM-LFIA platform also showed robust reproducibility and stability, maintaining consistent performance after four weeks of storage at 37 degrees C, equivalent to one year at 4 degrees C. Additionally, the smartphone application enables realtime quantitative analysis and cloud-based data management, enhancing usability for field applications and remote monitoring. These findings highlight the potential of this portable, low-cost multiplex detection system for rapid screening of respiratory viral antigens in diverse settings.
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